公开(公告)号：US20220099675A1

公开(公告)日：2022-03-31

申请号：US17489273

申请日：2021-09-29

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Xiahui CHEN ,  Shoukai KANG ,  Md Ashif IKBAL ,  Zhi ZHAO ,  Liangcai GU ,  Chao WANG

IPC: G01N33/569 ,  G01N33/543 ,  G01N21/78

Abstract: Provided herein are methods of detecting Ebola virus in a sample. The methods include contacting the sample with a plurality of gold nanoparticles (AuNPs) that are conjugated with at least two sets of antibodies, or antigen binding portions thereof, that binds to at least first or second epitopes of glycoproteins, such as secreted glycoproteins (sGPs) from the Ebola virus under conditions sufficient for the antibodies, or the antigen binding portions thereof, to bind to the first or second epitopes of the glycoproteins from the Ebola virus in the sample to produce bound glycoproteins. The methods also include detecting the glycoproteins from the Ebola virus when aggregations of the bound glycoproteins form with one another. Related reaction mixtures, devices, kits, and systems are also provided.

公开(公告)号：US20230375547A1

公开(公告)日：2023-11-23

申请号：US18247161

申请日：2021-09-29

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Xiahui CHEN ,  Shoukai KANG ,  MD Ashif IKBAL ,  Zhi ZHAO ,  Liangcai GU ,  Chao WANG

IPC: G01N33/569 ,  G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/54346 ,  G01N33/54386 ,  G01N2333/08 ,  G01N2469/10

Abstract: Provided herein are methods of detecting Ebola virus in a sample. The methods include contacting the sample with a plurality of gold nanoparticles (AuNPs) that are conjugated with antibodies, or antigen binding portions thereof, that bind to an epitope of a secreted glycoprotein (sGP) from the Ebola virus under conditions sufficient for the antibodies, or the antigen binding portions thereof, to bind to the epitope of the sGP from the Ebola virus in the sample to produce bound sGP. The methods also include detecting the sGP from the Ebola virus when aggregations of the bound sGP form with one another. Related reaction mixtures, devices, kits, and systems are also provided.