公开(公告)号：US20240100531A1

公开(公告)日：2024-03-28

申请号：US18274405

申请日：2022-01-28

申请人： Abbott Diagnostics Scarborough, Inc.

发明人： Austin M. Derfus ,  Neil A. Quitoviera ,  Adrian P. Crute ,  Carl G. Santavicca ,  Timothy L. Higby ,  Armando R. Tovar

IPC分类号： B01L3/00

CPC分类号： B01L3/523 ,  B01L3/502 ,  B01L2200/025 ,  B01L2300/044 ,  B01L2300/045 ,  B01L2300/0609 ,  B01L2300/0672 ,  B01L2300/0803 ,  B01L2300/0832 ,  B01L2400/0478

摘要： Aspects of the present disclosure include a buffer pack for dispensing buffer for sample preparation using a sample preparation cartridge. Also provided are a sealing plate configured for holding the buffer pack and a cap for actuating the buffer pack in the sample preparation cartridge. Aspects of the present disclosure include a sample preparation cartridge that includes the buffer pack and the sealing plate. Aspects of the present disclosure also include a sample preparation cartridge that includes the buffer pack, the sealing plate, and the cap. Methods for filling the buffer pack, assembling a cartridge for use in sample preparation, and using the cartridge for preparing a sample also provided.

公开(公告)号：US20240082845A1

公开(公告)日：2024-03-14

申请号：US18274395

申请日：2022-01-28

申请人： Abbott Diagnostics Scarborough, Inc.

发明人： Austin M. Derfus ,  Adrian P. Crute ,  Neil A. Quitoviera ,  Carl G. Santavicca ,  Timothy L. Higby ,  Karthikeyan Kumaravadivelu ,  Armando R. Tovar ,  Darren S. Gray ,  Torsten Gliewe

IPC分类号： B01L3/00

CPC分类号： B01L3/52 ,  B01L2200/0689 ,  B01L2300/042 ,  B01L2300/0832

摘要： Aspects of the present disclosure include a system for transporting a liquid from a chamber into one or more collection containers. The system may be semi-automatic or fully automatic. The system may be embodied in a sample preparation device, such as a cylindrical cartridge. The system includes a plunger chamber that creates negative pressure to fill the one or more collection containers. The plunger chamber is armed using a pivotable locking arm and a trigger coupled to the arm. Methods of using the system for transporting a liquid from a chamber into one or more collection containers is also provided.

公开(公告)号：US20240094099A1

公开(公告)日：2024-03-21

申请号：US18274325

申请日：2022-01-28

申请人： Abbott Diagnostics Scarborough, Inc.

发明人： Austin M. Derfus ,  Karthikeyan Kumaravadivelu ,  Kai Yang ,  Adrian P. Crute ,  Darren S. Gray ,  Armando R. Tovar ,  Tomoko Kobayashi ,  Tomotaka Komori ,  Yoshiko Wada ,  Masato Orikasa ,  Toru Yoshimura

IPC分类号： G01N1/34 ,  C12N15/10

CPC分类号： G01N1/34 ,  C12N15/1013

摘要： The methods for sample preparation disclosed herein utilize an air phase to reduce aqueous phase associated with magnetic particles bound to nucleic acids to decrease carryover of one or more contaminants, such as, cell debris, chaotropic agents, non-specifically attached molecules, and the like. This air-transfer step is improved by using a combination of a first population of magnetic particles and a second population of magnetic particles, where the first population of magnetic particles is capable of associating with nucleic acids, and the magnetic particles in the second population are at least two-times larger in size than the size of the magnetic particles in the first population. As discussed herein, the use of this second population of magnetic particles improves the transfer of the first population of magnetic particles by reducing loss of the first magnetic particles during the transfer. The sample preparation methods may be semi-automated or completely automated.