公开(公告)号：US5260195A

公开(公告)日：1993-11-09

申请号：US638263

申请日：1991-01-03

申请人： Abol F. Azhar ,  Arthur M. Usmani ,  Anthony D. Burke ,  Jill DuBois-Bousamra ,  Eric R. Diebold ,  Myron C. Rapkin ,  Mark T. Skarstedt

发明人： Abol F. Azhar ,  Arthur M. Usmani ,  Anthony D. Burke ,  Jill DuBois-Bousamra ,  Eric R. Diebold ,  Myron C. Rapkin ,  Mark T. Skarstedt

IPC分类号： C12N9/96 ,  C12N11/08 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/52

CPC分类号： C12N11/08 ,  G01N33/525 ,  Y10S435/805

摘要： Non-aqueous, polymeric reagent film compositions for use with analytical test devices of the dry chemistry type are described. The film compositions are prepared from an organic solution of a copolymer formed by interaction of at least two monomers wherein(a) the first monomer is a hydroxylated acrylate of the general formula ##STR1## where R1 is hydrogen or, more preferably, methyl, and R2 is a hydroxyalkyl group having from 1 to 5 carbon atoms, and(b) the second monomer is a neutral acrylate of the general formula ##STR2## where R1 is hydrogen or, more preferably, methyl, and R3 is a substituted or unsubstituted alkyl group having from 1 to 8 carbon atoms.Preferably, a third monomer is also included which is an amine-containing acrylate of the general formula ##STR3## where R1 is hydrogen or, more preferably, methyl, and R4 is a substituted or unsubstituted aminoalkyl or glycidyl group having from 1 to 5 carbon atoms or a surfactant polyethylene glycol group having from 10 to 30 carbon atoms. The film further comprises at least one enzyme in powder form dispersed therein and, optionally, a chromogenic indicator. The film composition is especially useful for determining components in body fluids, for example, for detecting glucose in whole blood, by rapid techniques.

摘要翻译： 描述了用于干化学类型的分析测试装置的非水性聚合物试剂膜组合物。 膜组合物由通过至少两种单体的相互作用形成的共聚物的有机溶液制备，其中（a）第一单体是通式为“IMAGE”的羟基化丙烯酸酯，其中R 1是氢或更优选甲基，和 R2是具有1至5个碳原子的羟烷基，和（b）第二单体是通式为“IMAGE”的中性丙烯酸酯，其中R 1是氢或更优选甲基，R 3是取代或未取代的烷基 具有1至8个碳原子的基团。 优选地，还包括第三单体，其是通式为“IMAGE”的含胺丙烯酸酯，其中R 1是氢或更优选甲基，R 4是具有1至5个碳的取代或未取代的氨基烷基或缩水甘油基 原子或具有10至30个碳原子的表面活性剂聚乙二醇基团。 该膜还包含分散在其中的至少一种粉末形式的酶，以及任选的显色指示剂。 通过快速技术，该膜组合物特别可用于测定体液中的组分，例如用于检测全血中的葡萄糖。