公开(公告)号：US5104811A

公开(公告)日：1992-04-14

申请号：US134950

申请日：1987-12-18

申请人： Dieter Berger ,  Wolfgang-Reinhold Knappe ,  Robert Lorenz ,  Henry M. Grage, Jr. ,  Mark T. Skarstedt ,  Bernward Sojka ,  Manfred Bleisteiner

发明人： Dieter Berger ,  Wolfgang-Reinhold Knappe ,  Robert Lorenz ,  Henry M. Grage, Jr. ,  Mark T. Skarstedt ,  Bernward Sojka ,  Manfred Bleisteiner

IPC分类号： G01N31/22 ,  C12M1/34 ,  G01N21/77 ,  G01N33/49 ,  G01N33/52 ,  G01N33/543 ,  G01N33/558

CPC分类号： G01N33/525 ,  G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/81

摘要： On a carrier layer there are arranged several test layers which are at least partly in fluid contact with one another, enabling liquid exchange. On the carrier layer are arranged an application zone, a detection zone for the production of a detectable signal characteristic of the analytical determination, which contains at least one said test layer which is a reaction layer, and an absorption zone with a test layer which is an absorptive layer of an absorbent material, wherein the reaction layer and absorption zone are positioned next to each other. Between the application and the absorption zone is a capillary-active transport path on the carrier layer which connects the application zone and the absorption zone. The reaction layer is arranged parallel to the transport path between the application zone and the absorption zone in such a manner that it is in liquid contact with a liquid transported in the transport path. The absorption zone therefore absorbs excess liquid and permits dosing the reaction layer with a reproducible amount of sample.

摘要翻译： 在载体层上布置有几个测试层，其至少部分地彼此流体接触，从而实现液体交换。 在载体层上布置有应用区域，用于产生分析测定特征的可检测信号的检测区域，其包含至少一个作为反应层的测试层，以及具有测试层的吸收区，其为 吸收材料的吸收层，其中反应层和吸收区彼此相邻放置。 在应用和吸收区之间是在载体层上的连接施加区和吸收区的毛细管 - 主动传输路径。 反应层以与输送路径中输送的液体液体接触的方式平行于施加区域和吸收区域之间的输送路径布置。 因此，吸收区吸收多余的液体，并允许用可重复量的样品给反应层。

公开(公告)号：US4683209A

公开(公告)日：1987-07-28

申请号：US887723

申请日：1986-07-21

申请人： Ibrahim A. Ismail ,  Paul Hemmes ,  Mark T. Skarstedt ,  Adam Zipp

发明人： Ibrahim A. Ismail ,  Paul Hemmes ,  Mark T. Skarstedt ,  Adam Zipp

IPC分类号： C12Q1/54 ,  G01N33/52 ,  G01N31/00

CPC分类号： G01N21/81 ,  C12Q1/54 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/104998

摘要： A viability test device wherein the viability of a test composition for the determination of an analyte of interest can be determined by simply wetting the device with ordinary water. The viability device can be prepared in three formats: a control or calibrator device, an internal control device and a self indicating device. Unreacted analyte, or an analog thereof, can be incorporated in a limited defined portion of a dried carrier matrix incorporated with a test composition to provide an internal control test device. When wetted with water, a positive optical response, usually color, indicates the test composition can provide a viable test for the analyte. The internal control test device is particularly advantageously used by diabetics in their own homes where a negative test response could be due to the desired control of the user's condition or due to deterioration of a test composition because of unfavorable storage conditions.

摘要翻译： 活力试验装置，其中用于测定感兴趣分析物的测试组合物的活力可通过简单地用普通水润湿该装置来确定。 活力装置可以以三种格式制备：控制或校准装置，内部控制装置和自我指示装置。 未反应的分析物或其类似物可以掺入含有测试组合物的干燥的载体基质的限定部分中以提供内部对照测试装置。 当用水润湿时，正光学响应（通常为颜色）表明测试组合物可以为分析物提供可行的测试。 内部控制测试装置特别有利地用于其本身的糖尿病患者，其中负面测试响应可能是由于对用户状况的期望控制或由于不利的储存条件而导致的测试组合物的劣化。

公开(公告)号：US5260195A

公开(公告)日：1993-11-09

申请号：US638263

申请日：1991-01-03

申请人： Abol F. Azhar ,  Arthur M. Usmani ,  Anthony D. Burke ,  Jill DuBois-Bousamra ,  Eric R. Diebold ,  Myron C. Rapkin ,  Mark T. Skarstedt

发明人： Abol F. Azhar ,  Arthur M. Usmani ,  Anthony D. Burke ,  Jill DuBois-Bousamra ,  Eric R. Diebold ,  Myron C. Rapkin ,  Mark T. Skarstedt

IPC分类号： C12N9/96 ,  C12N11/08 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/52

CPC分类号： C12N11/08 ,  G01N33/525 ,  Y10S435/805

摘要： Non-aqueous, polymeric reagent film compositions for use with analytical test devices of the dry chemistry type are described. The film compositions are prepared from an organic solution of a copolymer formed by interaction of at least two monomers wherein(a) the first monomer is a hydroxylated acrylate of the general formula ##STR1## where R1 is hydrogen or, more preferably, methyl, and R2 is a hydroxyalkyl group having from 1 to 5 carbon atoms, and(b) the second monomer is a neutral acrylate of the general formula ##STR2## where R1 is hydrogen or, more preferably, methyl, and R3 is a substituted or unsubstituted alkyl group having from 1 to 8 carbon atoms.Preferably, a third monomer is also included which is an amine-containing acrylate of the general formula ##STR3## where R1 is hydrogen or, more preferably, methyl, and R4 is a substituted or unsubstituted aminoalkyl or glycidyl group having from 1 to 5 carbon atoms or a surfactant polyethylene glycol group having from 10 to 30 carbon atoms. The film further comprises at least one enzyme in powder form dispersed therein and, optionally, a chromogenic indicator. The film composition is especially useful for determining components in body fluids, for example, for detecting glucose in whole blood, by rapid techniques.

摘要翻译： 描述了用于干化学类型的分析测试装置的非水性聚合物试剂膜组合物。 膜组合物由通过至少两种单体的相互作用形成的共聚物的有机溶液制备，其中（a）第一单体是通式为“IMAGE”的羟基化丙烯酸酯，其中R 1是氢或更优选甲基，和 R2是具有1至5个碳原子的羟烷基，和（b）第二单体是通式为“IMAGE”的中性丙烯酸酯，其中R 1是氢或更优选甲基，R 3是取代或未取代的烷基 具有1至8个碳原子的基团。 优选地，还包括第三单体，其是通式为“IMAGE”的含胺丙烯酸酯，其中R 1是氢或更优选甲基，R 4是具有1至5个碳的取代或未取代的氨基烷基或缩水甘油基 原子或具有10至30个碳原子的表面活性剂聚乙二醇基团。 该膜还包含分散在其中的至少一种粉末形式的酶，以及任选的显色指示剂。 通过快速技术，该膜组合物特别可用于测定体液中的组分，例如用于检测全血中的葡萄糖。

公开(公告)号：US4649121A

公开(公告)日：1987-03-10

申请号：US705711

申请日：1985-02-26

申请人： Ibrahim A. Ismail ,  Paul Hemmes ,  Mark T. Skarstedt ,  Adam Zipp

发明人： Ibrahim A. Ismail ,  Paul Hemmes ,  Mark T. Skarstedt ,  Adam Zipp

IPC分类号： G01N33/66 ,  C12Q1/54 ,  G01N21/78 ,  G01N31/22 ,  G01N33/50 ,  G01N33/52 ,  G01N35/00

CPC分类号： G01N33/521 ,  C12Q1/54 ,  Y10T436/104998

摘要： A viability test device wherein the viability of a test composition for the determination of an analyte of interest can be determined by simply wetting the device with ordinary water. The viability device can be prepared in three formats: a control or calibrator device, an internal control device and a self indicating device. Unreacted analyte, or an analog thereof, can be incorporated in a limited defined portion of a dried carrier matrix incorporated with a test composition to provide an internal control test device. When wetted with water, a positive optical response, usually color, indicates the test composition can provide a viable test for the analyte. The internal control test device is particularly advantageously used by diabetics in their own homes where a negative test response could be due to the desired control of the user's condition or due to deterioration of a test composition because of unfavorable storage conditions.

公开(公告)号：US4141688A

公开(公告)日：1979-02-27

申请号：US823618

申请日：1977-08-11

申请人： David A. N. Morris ,  Patricia A. Rupchock ,  Mark T. Skarstedt

发明人： David A. N. Morris ,  Patricia A. Rupchock ,  Mark T. Skarstedt

IPC分类号： C07D241/38 ,  C07D241/46 ,  C07D265/34 ,  C07D265/38 ,  C07D279/18 ,  C09B19/00 ,  C09B21/00 ,  C09B67/32 ,  G01N31/00 ,  G01N33/52 ,  G01N33/82 ,  C07D279/34 ,  G01N31/22

CPC分类号： C07D241/38 ,  C07D241/46 ,  C07D265/34 ,  C07D265/38 ,  C07D279/18 ,  C09B19/00 ,  C09B21/00 ,  C09B67/0075 ,  G01N33/52 ,  G01N33/82 ,  Y10S436/904 ,  Y10T436/142222 ,  Y10T436/201666

摘要： A composition, device and method for determining the presence of a reducing agent, such as ascorbic acid, in a test sample are disclosed. The composition comprises certain thiazine and oxazine compounds which, in their oxidized state, are colored, but which become colorless when reduced by a reducing agent. The device comprises a carrier matrix incorporated with the composition, and the method comprises contacting a test sample with the composition device and observing any detectable response such as a color change.

摘要翻译： 公开了用于测定样品中还原剂如抗坏血酸的存在的组合物，装置和方法。 该组合物包含某些噻嗪和恶嗪化合物，它们的氧化状态为着色，但当被还原剂还原时变成无色。 该装置包括与组合物结合的载体基质，并且该方法包括使测试样品与组合物装置接触并观察任何可检测的响应，例如颜色变化。