公开(公告)号：US09005897B2

公开(公告)日：2015-04-14

申请号：US13788627

申请日：2013-03-07

Applicant: Agency for Science, Technology and Research

Inventor： Sai Kiang Lim ,  Elias Lye

IPC: C12Q1/68 ,  C12N5/071 ,  G01N33/50 ,  C12N5/0775

CPC classification number: C12N5/0603 ,  C12N5/0662 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/734 ,  C12N2502/1352 ,  C12N2506/02 ,  C12Q1/6809 ,  G01N33/5044

Abstract: We disclose a method comprising: (a) providing an embryonic stem (ES) cell; and (b) establishing a progenitor cell line from the embryonic stem cell; in which the progenitor cell line is selected based on its ability to self-renew. Preferably, the method selects against somatic cells based on their inability to self-renew. Preferably, the progenitor cell line is derived or established in the absence of co-culture, preferably in the absence of feeder cells, which preferably selects against embryonic stem cells. Optionally, the method comprises (d) deriving a differentiated cell from the progenitor cell line.