公开(公告)号：US20240344037A1

公开(公告)日：2024-10-17

申请号：US18761109

申请日：2024-07-01

申请人： Genesis Technologies Limited

发明人： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

IPC分类号： C12N5/074 ,  C12N5/071 ,  C12N5/0775 ,  C12N5/0789 ,  C12N5/0797 ,  C12N15/85

CPC分类号： C12N5/0696 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0662 ,  C12N5/0678 ,  C12N15/85 ,  C12N2501/40 ,  C12N2501/60 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/998 ,  C12N2502/99 ,  C12N2506/094 ,  C12N2506/11 ,  C12N2506/1307 ,  C12N2506/1384

摘要： Described herein are reprogrammed cells, and methods for cell dedifferentiation, transformation and eukaryotic cell reprogramming. Also described are cells, cell lines, and tissues that can be transplanted in a patient after steps of in vitro dedifferentiation and in vitro reprogramming. In particular embodiments the cells are Stem-Like Cells (SLCs), including Neural Stem-Like Cells (NSLCs), Cardiac Stem-Like Cells (CSLC), Hematopoietic Stem-Like Cells (HSLC), Pancreatic Progenitor-Like Cells, and Mesendoderm-like Cells. Also described are methods for generating these cells from human somatic cells and other types of cells. Also provided are compositions and methods of using of the cells so generated in human therapy and in other areas.

公开(公告)号：US12115194B2

公开(公告)日：2024-10-15

申请号：US17964905

申请日：2022-10-12

申请人： Mesoblast International Sarl

发明人： Silviu Itescu ,  Lee Golden

IPC分类号： A61K35/28 ,  A61K9/00 ,  A61K35/12 ,  C12N5/0775

CPC分类号： A61K35/28 ,  C12N5/0662 ,  A61K9/0019 ,  A61K2035/124 ,  A61K2300/00

摘要： The present disclosure relates to methods for preventing progressive heart failure in subjects with persistent left ventricular (LV) dysfunction. Such methods may also be used for treating or preventing progressive heart failure in subjects with a proximal left anterior descending (LAD) lesion and persistent left ventricular dysfunction.

公开(公告)号：US20240301356A1

公开(公告)日：2024-09-12

申请号：US18484347

申请日：2023-10-10

申请人： MESOBLAST INTERNATIONAL SÁRL

发明人： Alla DANILKOVITCH ,  Diane CARTER ,  Alicia Mildred TYRELL ,  Simon BUBNIC ,  Michelle MARCELINO ,  Rodney MONROY

IPC分类号： C12N5/0775 ,  A61K35/12 ,  A61K35/28

CPC分类号： C12N5/0662 ,  A61K35/28 ,  C12N5/0663 ,  A61K2035/122 ,  C12N2501/25 ,  G01N2333/525

摘要： Mesenchymal stem cells which express TNF-α receptor Type I in an amount of at least 13 pg/106 cells. Such mesenchymal stem cells inhibit the proliferation of lymphocytes and may be employed, in particular, in the treatment of graft-versus-host disease.

公开(公告)号：US12084684B2

公开(公告)日：2024-09-10

申请号：US17259508

申请日：2019-07-15

申请人： The Board of Trustees of the Leland Stanford Junior University ,  UNIVERSITY OF WASHINGTON

发明人： Kenan Christopher Garcia ,  Yi Miao ,  David Baker ,  Keunwan Park ,  Luke Dang

IPC分类号： C12N5/0775 ,  C07K14/47 ,  C12N5/071

CPC分类号： C12N5/0662 ,  C07K14/4703 ,  C07K14/4705 ,  C12N5/0697 ,  C07K2319/74 ,  C12N2501/415

摘要： Wnt signaling antagonist and agonist compositions and methods for their use are provided.

公开(公告)号：US12042791B2

公开(公告)日：2024-07-23

申请号：US15958976

申请日：2018-04-20

申请人： Cedars-Sinai Medical Center

发明人： Dan Gazit ,  Gadi Pelled ,  Zulma Gazit ,  Dmitriy Sheyn

IPC分类号： C12N5/00 ,  A61K49/00 ,  A61K51/02 ,  A61L27/38 ,  B01L3/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0775 ,  G01N33/50 ,  G01T1/164 ,  G01T1/29 ,  C07K14/78 ,  C12M1/34

CPC分类号： B01L3/5027 ,  A61K49/0019 ,  A61K49/005 ,  A61K51/02 ,  A61L27/3895 ,  B01L3/5085 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0668 ,  C12N5/0696 ,  C12N5/0697 ,  G01N33/5038 ,  G01T1/1644 ,  G01T1/2985 ,  A61M2205/3334 ,  C07K14/78 ,  C12M41/40

摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

公开(公告)号：US20240233867A1

公开(公告)日：2024-07-11

申请号：US18412092

申请日：2024-01-12

申请人： FUJIFILM Corporation

发明人： Kentaro NAKAMURA ,  Yuichi YOSHINO ,  Rie HANDO

IPC分类号： G16B25/10 ,  A61K35/28 ,  C12N5/0775

CPC分类号： G16B25/10 ,  A61K35/28 ,  C12N5/0662

摘要： An object of the present invention is to provide such a quality management method for a cell that enables the sorting-out of a cell having drug efficacy, and a method of producing a cell using the quality management method. According to the present invention, there is provided a method that is a quality management method for a specific cell, where the method includes evaluating a correlation relationship between an expression pattern of a gene group obtained by measuring an expression state of a related gene group that is characteristic of the specific cell and an expression pattern that serves as a reference and determining the cell to be usable in a case where the correlation relationship is equal to or higher than a certain level.

公开(公告)号：US20240216438A1

公开(公告)日：2024-07-04

申请号：US18561930

申请日：2022-05-18

申请人： SCM LIFESCIENCE CO., LTD.

发明人： Sun Uk SONG ,  Si Na KIM ,  Byeol CHOI

IPC分类号： A61K35/28 ,  A61K8/98 ,  A61P17/00 ,  A61Q19/00 ,  C12N5/0775

CPC分类号： A61K35/28 ,  A61K8/981 ,  A61P17/00 ,  A61Q19/00 ,  C12N5/0662

摘要： The present invention relates to a composition containing primed, function-reinforced stem cells for preventing, alleviating or treating atopic dermatitis. The stem cells primed by treatment with TNF-α, IFN-γ, and IFN-α or with TNF-α, IFN-γ, IFN-α, and vitamins according to the present invention have reinforced anti-inflammatory and immunomodulatory ability and thus exhibit excellent prophylactic or therapeutic effects on atopic dermatitis symptoms, compared to stem cells that are not treated with the above priming factors. Therefore, the function-reinforced stem cells of the present invention can be widely utilized in various fields of prevention, alleviation, or treatment of atopic dermatitis.

公开(公告)号：US20240209321A1

公开(公告)日：2024-06-27

申请号：US18496419

申请日：2023-10-27

申请人： President and Fellows of Harvard College

发明人： Hon Man Alex Ng ,  George M. Church ,  Volker Busskamp

IPC分类号： C12N5/071 ,  C12N5/073 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/0797

CPC分类号： C12N5/069 ,  C12N5/0605 ,  C12N5/0623 ,  C12N5/0626 ,  C12N5/0658 ,  C12N5/0662 ,  C12N5/0686 ,  C12N5/0696 ,  C12N2501/60 ,  C12N2503/02 ,  C12N2506/45 ,  C12N2510/00

摘要： Forced expression of a handful of transcription factors (TFs) can induce conversions between cell identities; however, the extent to which TFs can alter cell identity has not been systematically assessed. Here, we assembled a human TFome, a comprehensive expression library of 1,578 human TF clones with full coverage of the major TF families. By systematically screening the human TFome, we identified 77 individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression profiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.

公开(公告)号：US20240156083A1

公开(公告)日：2024-05-16

申请号：US18506291

申请日：2023-11-10

申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Yu Shrike Zhang ,  Zeyu Luo

IPC分类号： A01N1/02 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/079

CPC分类号： A01N1/0284 ,  A01N1/0221 ,  C12N5/0618 ,  C12N5/0656 ,  C12N5/0658 ,  C12N5/0662 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2537/10

摘要： A method of making a 3D-printed biomaterial is described. The method includes depositing a hydrogel comprising cells and a cryoprotectant from a 3D printer onto a freezing plate to form a frozen hydrogel filament; depositing additional hydrogel comprising cells and a cryoprotectant from a 3D printer in contact with a previously placed frozen hydrogel filament to form a 3D-printed biomaterial comprising a plurality of frozen hydrogel filaments; and removing the 3D-printed biomaterial from the freezing plate. 3D-printed biomaterials made by the method are also described.

公开(公告)号：US20240150719A1

公开(公告)日：2024-05-09

申请号：US18547408

申请日：2022-02-21

申请人： THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

发明人： Brijesh S. GILL ,  Charles S. COX, Jr.

IPC分类号： C12N5/0775 ,  C12M1/42 ,  C12M3/00 ,  C12M3/04 ,  C12N5/00 ,  C12N13/00

CPC分类号： C12N5/0662 ,  C12M23/48 ,  C12M27/10 ,  C12M35/04 ,  C12M35/06 ,  C12N5/0075 ,  C12N13/00 ,  C12N2527/00

摘要： Apparatus and methods for culturing cells in a cell culture medium including magnetic beads in a fluid. A variable magnetic field can be applied to the magnetic beads to create shear forces on cells on the surface of the beads. In certain embodiments, a rotational force can also be applied to the magnetic beads and the magnetic force can counteract the rotational force.