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    Nanoprobe-based genetic testing
    2.
    发明授权
    Nanoprobe-based genetic testing 有权

    公开(公告)号：US10214776B2

    公开(公告)日：2019-02-26

    申请号：US14783392

    申请日：2014-04-04

    Applicant: Agency for Science, Technology and Research

    Inventor： Yanbing Zu ,  Jackie Y. Ying

    IPC: C12Q1/6827 ,  C12Q1/6883 ,  B82Y15/00

    Abstract: The present application relates to methods of detecting a mutation in a target nucleic acid molecule. Two phosphorodiamidate morpholino oligomer probes that differ by at least one base are each covalently coupled to a nano article and hybridized to a target sequence. The melting temperature of the complexes between each of the two probes and the target nucleic acid are measured and compared to determine whether the sample contains a nucleic acid with the mutation. Further, the present invention relates to kits comprising a first and second conjugate as described herein and to the use of such kits for the detection of mutations in a target nucleic acid molecule or for assigning a genotype to a target nucleic acid molecule.

    DETERMINATION OF NUCLEIC ACID METHYLATION
    3.
    发明申请
    DETERMINATION OF NUCLEIC ACID METHYLATION 审中-公开

    公开(公告)号：US20190017110A1

    公开(公告)日：2019-01-17

    申请号：US15778876

    申请日：2016-11-25

    Applicant: Agency for Science, Technology and Research

    Inventor： Yanbing Zu ,  Jackie Y. Ying

    IPC: C12Q1/6858 ,  C12Q1/6886

    Abstract: The invention relates to methods and kits for determining the methylation status of a target nucleic acid molecule in a sample comprising (a) bisulfite treatment of the target nucleic acid, (b) amplifying the treated target nucleic acid with methylation-specific primers, (c) contacting the amplicons with a methylation-specific plasmonic nanoprobe and (d) determining the methylation status based on melting temperature Tm of the hybrid probe and the target nucleic acid. In particular, a plasmonic gold nanoparticle covalently coupled to a morpholino oligonucleotide probe. Also claimed are methods and kits for determining methylation status of the Septin 9 (SEPT9) gene promoter.

    Determination of nucleic acid methylation
    6.
    发明授权
    Determination of nucleic acid methylation 有权

    公开(公告)号：US11021743B2

    公开(公告)日：2021-06-01

    申请号：US15778876

    申请日：2016-11-25

    Applicant: Agency for Science, Technology and Research

    Inventor： Yanbing Zu ,  Jackie Y. Ying

    IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6886

    Abstract: The invention relates to methods and kits for determining the methylation status of a target nucleic acid molecule in a sample comprising (a) bisulfite treatment of the target nucleic acid, (b) amplifying the treated target nucleic acid with methylation-specific primers, (c) contacting the amplicons with a methylation-specific plasmonic nanoprobe and (d) determining the methylation status based on melting temperature Tm of the hybrid probe and the target nucleic acid. In particular, a plasmonic gold nanoparticle covalently coupled to a morpholino oligonucleotide probe. Also claimed are methods and kits for determining methylation status of the Septin 9 (SEPT9) gene promoter.

    DETERMINATION OF MTRNR1 GENE MUTATION
    8.
    发明申请
    DETERMINATION OF MTRNR1 GENE MUTATION 审中-公开

    公开(公告)号：US20200165672A1

    公开(公告)日：2020-05-28

    申请号：US15999372

    申请日：2017-02-09

    Applicant: Agency for Science, Technology and Research

    Inventor： Yanbing Zu ,  Jackie Y. Ying

    IPC: C12Q1/6883

    Abstract: The invention relates to methods and kits for determining the presence of a mutation selected from the group consisting of 1555A>G and 1494C>T in human Mitochondrially Encoded 12S RNA (MTRNR1) gene in a sample from a subject, comprising, inter alia, amplifying at least part of the MTRNR1 gene and detecting the amplified DNA with a plasmonic gold nanoparticle covalently coupled to a morpholino oligonucleotide probe. The claimed method may be used for determining the risk of a subject to Aminoglycoside-induced hearing loss.

    NANOPROBE-BASED GENETIC TESTING
    10.
    发明申请
    NANOPROBE-BASED GENETIC TESTING 审中-公开
    基于NANOPROBE的遗传测试

    公开(公告)号：US20160046993A1

    公开(公告)日：2016-02-18

    申请号：US14783392

    申请日：2014-04-04

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： Yanbing Zu ,  Jackie Y. Ying

    IPC: C12Q1/68

    CPC classification number: C12Q1/6883 ,  B82Y15/00 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/113 ,  C12Q2527/107 ,  C12Q2563/155 ,  C12Q2565/519

    Abstract: The present application relates to methods of detecting a mutation in a target nucleic acid molecule. Two phosphorodiamidate morpholino oligomer probes that differ by at least one base are each covalently coupled to a nano article and hybridised to a target sequence. The melting temperature of the complexes between each of the two probes and the target nucleic acid are measured and compared to determine whether the sample contains a nucleic acid with the mutation. Further, the present invention relates to kits comprising a first and second conjugate as described herein and to the use of such kits for the detection of mutations in a target nucleic acid molecule or for assigning a genotype to a target nucleic acid molecule.

    Abstract translation: 本申请涉及检测靶核酸分子中的突变的方法。 两个不同于至少一个碱基的二亚磷酰氯吗啉代低聚物探针各自共价偶联到纳米制品上并与目标序列杂交。 测量两个探针和靶核酸中的每一个之间的络合物的熔解温度并进行比较，以确定样品是否含有具有突变的核酸。 此外，本发明涉及包含如本文所述的第一和第二缀合物的试剂盒以及用于检测靶核酸分子中的突变或用于将靶基因分配给靶核酸分子的用途。

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