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公开(公告)号：US11506581B2
公开(公告)日：2022-11-22
申请号：US16290322
申请日：2019-03-01
Applicant: Agilent Technologies, Inc.
Inventor： Genevieve Van de Bittner
Abstract: Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.
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公开(公告)号：US20190285522A1
公开(公告)日：2019-09-19
申请号：US16290053
申请日：2019-03-01
Applicant: Agilent Technologies, Inc.
Inventor： Genevieve Van de Bittner , Brian Phillip Smart
Abstract: Methods for removing salts from a metabolite solution. The methods comprise forming an insoluble silver phosphate salt. Further methods include methods for removing hydrolyzed fluorous compounds. These methods comprise extraction with a fluorous solvent in the presence of a protonation reagent and/or chromatography on a fluorous affinity resin. Methods also include separating lysed cell debris and denatured proteins/disrupted enzymes from a metabolite mixture in a container with a filter, where live cells are grown prior to the lysis, either adherent to the filter or in suspension above the filter. The cells are then lysed in the container.
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公开(公告)号：US11808675B2
公开(公告)日：2023-11-07
申请号：US16874499
申请日：2020-05-14
Applicant: Agilent Technologies, Inc.
Inventor： Genevieve Van de Bittner , James Alexander Apffel , Steven M. Fischer , Christine A. Miller , Kristin Briana Bernick
CPC classification number: G01N1/28 , B01D11/0288 , B01D15/00 , C07K1/145 , C12N1/06 , C12N15/1006
Abstract: Reagents and methods for extracting and stabilizing metabolites at room temperature and methods in which metabolites and one or more of proteins, lipids and nucleic acids are extracted from a single sample in a unified workflow.
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公开(公告)号：US20190293530A1
公开(公告)日：2019-09-26
申请号：US16290322
申请日：2019-03-01
Applicant: Agilent Technologies, Inc.
Inventor： Genevieve Van de Bittner
Abstract: Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.
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