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1.发明授权Method for accumulation of polyhydroxyalkanoates in biomass with on-line monitoring for feed rate control and process termination 失效多羟基链烷酸酯在生物质中积累的方法，在线监测进料速率控制和过程终止公开(公告)号：US08748138B2
公开(公告)日：2014-06-10
申请号：US13514660
申请日：2010-12-10
摘要： A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.
摘要翻译： 用于在生物质中生产聚羟基链烷酸酯（PHA）的方法或方法。 该方法需要向含有PH的积累细菌的生物质中加入含有机碳的底物。 特别地，该方法需要在所选择的时间段内至少三次分开地间歇地向生物质供应底物。 该方法的目的是产生具有相对较高分子量，至少400,000g / mol的PHA。 通过控制将基底供应给生物质的频率并通过将足够量的底物供入生物质，该方法或方法产生具有相对较高分子量的PHA。
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2.发明申请Method of Treating Wastewater and Producing an Activated Sludge Having a High Biopolymer Production Potential 审中-公开处理废水和生产具有高生物聚合物生产潜力的活性污泥的方法公开(公告)号：US20120305478A1
公开(公告)日：2012-12-06
申请号：US13516760
申请日：2010-07-29
申请人： Alan Gideon Werker , Simon Olof Harald Bengtsson , Fernando Morgan-Sagastume , Carl Anton Börje Karlsson , Elise Marie Blanchet
发明人： Alan Gideon Werker , Simon Olof Harald Bengtsson , Fernando Morgan-Sagastume , Carl Anton Börje Karlsson , Elise Marie Blanchet
CPC分类号： C12P7/625 , C02F1/24 , C02F3/1263 , C02F3/34 , C02F2203/004 , C02F2209/21 , C02F2209/22 , Y02W10/15 , Y02W10/45
摘要： A method or process is provided for treating wastewater and producing a polyhydroxyalkanote (PHA)-storing biomass. The method or process entails biologically treating wastewater and in one process a filamentous biomass is selected and caused to proliferate so as to dominate an activated sludge. The filamentous biomass is utilized to treat the wastewater and to remove contaminants therefrom. As a part of this process, there is provided an enhancement for PHA production potential in the said biomass. This entails enhancing the PHA production potential of the filamentous biomass by subjecting the biomass to alternating feast and famine conditions where under feast conditions more biodegradable organic substrate is available to the filamentous biomass than under famine conditions. In another process, wastewater is treated with an activated sludge. The wastewater is treated in a main stream and as a part of the process, the activated sludge and biomass contained therein is concentrated and directed to a side stream. In the side stream, at least a portion of the enhancement for PHA production potential in the biomass from the process is carried out. In one particular process, the activated sludge and the biomass contained therein is concentrated by a separator and the concentrated biomass is directed to a side stream and subjected to famine conditions.
摘要翻译： 提供了一种用于处理废水并生产聚羟基卡那酮（PHA）的生物质的方法或方法。 该方法或方法需要生物处理废水，并且在一个过程中，丝状生物质被选择并引起增殖以便主导活性污泥。 丝状生物质用于处理废水并从中除去污染物。 作为该过程的一部分，提供了在所述生物质中PHA生产潜力的增强。 这需要通过使生物质经历交替的盛宴和饥荒条件来提高丝状生物质的PHA生产潜力，其中在盛宴条件下，在饥荒条件下，丝状生物质可获得更可生物降解的有机底物。 在另一个过程中，废水用活性污泥处理。 废水在主流中进行处理，作为该方法的一部分，其中所含的活性污泥和生物质被浓缩并引导至侧流。 在侧流中，进行来自该方法的生物质中PHA生产潜力的增强的至少一部分。 在一个特定的方法中，活性污泥及其中所含的生物质通过分离器浓缩，浓缩的生物质被引导至侧流并经历饥荒条件。
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3.发明申请Method of Treating Municipal Wastewater and Producing Biomass with Biopolymer Production Potential 审中-公开用生物聚合物生产潜力处理城市污水和生产生物质的方法公开(公告)号：US20130199997A1
公开(公告)日：2013-08-08
申请号：US13817021
申请日：2011-08-17
申请人： Alan Gideon Werker , Simon Olof Harald Bengtsson , Elise Marie Blanchet , Maria das Dores Tavares Gurgo e Cirne , Carl Anton Börje Karlsson , Fernando Morgan-Sagastume
发明人： Alan Gideon Werker , Simon Olof Harald Bengtsson , Elise Marie Blanchet , Maria das Dores Tavares Gurgo e Cirne , Carl Anton Börje Karlsson , Fernando Morgan-Sagastume
IPC分类号： C02F3/12
CPC分类号： C02F3/1221 , C02F3/12 , C02F3/1263 , C02F2203/004 , C02F2209/10 , C02F2209/21 , Y02W10/15 , Y02W10/45
摘要： A method of biologically treating wastewater and removing contaminants from the wastewater is disclosed. In the course of treating the wastewater, biomass is produced. In addition to removing contaminants from the wastewater, the process or method of the present invention entails enhancing the PHA accumulation potential of the biomass. Disclosed are a number of processes that are employed in a biological wastewater treatment system for enhancing PHA accumulation potential. For example, enhanced PHA accumulation potential is realized by exposing the biomass to feast and famine conditions and, after exposing the biomass to famine conditions, stimulating the biomass into a period of feast by exposing the biomass to feast conditions for a selected period of time by applying an average peak stimulating RBCOD feeding rate of greater than 5 mg-COD\L\MIN in combination with an average peak specific RBCOD feeding rate greater than 0.5 mg-COD\g-VSS\MIN. In another example, the PHA accumulation potential of the biomass is enhanced by subjecting the biomass to feast conditions that cause the biomass to reach a peak respiration rate that is at least 40% of the extant maximum respiration rate of the biomass. Other processes are discussed that can contribute to enhancing PHA accumulation potential of biomass.
摘要翻译： 公开了生物处理废水并从废水中去除污染物的方法。 在处理废水的过程中，生产生物质。 除了从废水中除去污染物外，本发明的方法或方法需要提高生物质的PHA积累潜力。 公开了用于提高PHA积累潜力的生物废水处理系统中的许多方法。 例如，增强的PHA积累潜力是通过将生物量暴露于饥饿和饥荒条件下实现的，并且在将生物质暴露于饥荒条件之后，通过将生物质暴露于选定时间段的盛宴条件来将生物质刺激到一段盛宴期间 应用平均峰值刺激RBCOD摄入率大于5 mg-COD \ L \ MIN与平均峰值特异性RBCOD摄入率大于0.5 mg-COD \ g-VSS \ MIN。 在另一个实例中，生物质的PHA积累潜力通过使生物质经历使得生物质达到至少40％的生物量最大呼吸速率的峰值呼吸速率的条件来增强。 讨论了有助于提高生物质PHA积累潜力的其他过程。
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4.发明申请Method for Accumulation of Polyhydroxyalkanoates in Biomass with On-Line Monitoring for Feed Rate Control and Process Termination 失效用于在饲料速率控制和过程终止中在线监测生物质聚羟基链烷酸酯的方法公开(公告)号：US20130029388A1
公开(公告)日：2013-01-31
申请号：US13514660
申请日：2010-12-10
IPC分类号： C12P7/62
摘要： A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.
摘要翻译： 用于在生物质中生产聚羟基链烷酸酯（PHA）的方法或方法。 该方法需要向含有PH的积累细菌的生物质中加入含有机碳的底物。 特别地，该方法需要在所选择的时间段内至少三次分开地间歇地向生物质供应底物。 该方法的目的是产生具有相对较高分子量，至少400,000g / mol的PHA。 通过控制将基底供应给生物质的频率并通过将足够量的底物供入生物质，该方法或方法产生具有相对较高分子量的PHA。
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5.发明申请公开(公告)号：US20100200498A1
公开(公告)日：2010-08-12
申请号：US12704732
申请日：2010-02-12
申请人： Simon Olof Harald Bengtsson , Alan Gideon Werker , Paulo Alexandre da Costa Lemos , Maria Ascencao Carvalho Fernandes de Miranda Reis
发明人： Simon Olof Harald Bengtsson , Alan Gideon Werker , Paulo Alexandre da Costa Lemos , Maria Ascencao Carvalho Fernandes de Miranda Reis
IPC分类号： C02F3/30
摘要： A process is provided for increasing the production of PHA in a mixed culture biomass. In a first stage of the process, organic material associated with a substrate is converted to volatile fatty acids. In the case of a wastewater treatment process, if the wastewater includes sufficient volatile fatty acids (VFAs) to support the process, then it is unnecessary to convert organic material to VFAs. In a second stage of the process, an anaerobic-aerobic selection process is utilized to select glycogen accumulating organisms that cause these organisms to proliferate and dominate the open mixed culture biomass. By providing relatively high organic loading in the form of VFAs in the anaerobic treatment phase of the selection process, glycogen accumulating organisms having a relatively high level of stored glycogen are produced. In a third stage, the PHA accumulation process is practiced where the glycogen rich organisms are fed VFAs under anaerobic or aerobic conditions or combinations thereof. Through the consumption of externally supplied VFAs and internally stored glycogen, relatively high levels of PHA in the biomass are produced. Thereafter PHA is separated from the residual biomass.
摘要翻译： 提供了一种用于增加混合培养生物质中PHA生产的方法。 在该方法的第一阶段，与底物相关的有机材料转化成挥发性脂肪酸。 在废水处理方法的情况下，如果废水含有足够的挥发性脂肪酸（VFA）来支持该过程，则不必将有机物质转化为VFA。 在该方法的第二阶段中，利用厌氧 - 好氧选择方法来选择使这些生物体增殖并主导开放混合培养生物质的糖原积累生物体。 通过在选择过程的厌氧处理阶段提供相对高的有机负荷的形式，产生具有相对高水平储存的糖原的糖原积累生物体。 在第三阶段中，在富含糖原的生物体在厌氧或需氧条件下或其组合下进料VFA时，实施PHA积累过程。 通过消耗外部供应的VFAs和内部储存的糖原，生产生物质中相对较高水平的PHA。 此后，PHA与剩余生物量分离。
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6.发明授权公开(公告)号：US08187462B2
公开(公告)日：2012-05-29
申请号：US12704732
申请日：2010-02-12
申请人： Simon Olof Harald Bengtsson , Alan Gideon Werker , Paulo Alexandre da Costa Lemos , Maria Ascensao Carvalho Fernandes de Miranda Reis
发明人： Simon Olof Harald Bengtsson , Alan Gideon Werker , Paulo Alexandre da Costa Lemos , Maria Ascensao Carvalho Fernandes de Miranda Reis
IPC分类号： C02F3/30
摘要： A process is provided for increasing the production of PHA in a mixed culture biomass. In a first stage of the process, organic material associated with a substrate is converted to volatile fatty acids. In the case of a wastewater treatment process, if the wastewater includes sufficient volatile fatty acids (VFAs) to support the process, then it is unnecessary to convert organic material to VFAs. In a second stage of the process, an anaerobic-aerobic selection process is utilized to select glycogen accumulating organisms that cause these organisms to proliferate and dominate the open mixed culture biomass. By providing relatively high organic loading in the form of VFAs in the anaerobic treatment phase of the selection process, glycogen accumulating organisms having a relatively high level of stored glycogen are produced. In a third stage, the PHA accumulation process is practiced where the glycogen rich organisms are fed VFAs under anaerobic or aerobic conditions or combinations thereof. Through the consumption of externally supplied VFAs and internally stored glycogen, relatively high levels of PHA in the biomass are produced. Thereafter PHA is separated from the residual biomass.
摘要翻译： 提供了一种用于增加混合培养生物质中PHA生产的方法。 在该方法的第一阶段，与底物相关的有机材料转化成挥发性脂肪酸。 在废水处理方法的情况下，如果废水含有足够的挥发性脂肪酸（VFA）来支持该过程，则不必将有机物质转化为VFA。 在该方法的第二阶段中，利用厌氧 - 好氧选择方法来选择使这些生物体增殖并主导开放混合培养生物质的糖原积累生物体。 通过在选择过程的厌氧处理阶段提供相对高的有机负荷的形式，产生具有相对高水平储存的糖原的糖原积累生物体。 在第三阶段中，在富含糖原的生物体在厌氧或需氧条件下或其组合下进料VFA时，实施PHA积累过程。 通过消耗外部供应的VFAs和内部储存的糖原，生产生物质中相对较高水平的PHA。 此后，PHA与剩余生物量分离。
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公开(公告)号：US20170233271A1
公开(公告)日：2017-08-17
申请号：US15502052
申请日：2015-08-06
申请人： Alan Gideon WERKER , Simon Olof Harald BENGTSSON , John Markus HJORT , Fernando MORGAN-SAGASTUME , Mauro MAJONE , Francesco VALENTINO , VEOLIA WATER SOLUTIONS & TECHNOLOGIES SUPPORT , UNIVERSITY OF ROME
发明人： Alan Gideon Werker , Simon Olof Harald Bengtsson , John Markus Hjort , Fernando Morgan-Sagastume , Mauro Majone , Francesco Valentino
CPC分类号： C02F3/1205 , C02F3/1263 , C02F3/34 , C02F2203/004 , C02F2209/06 , C02F2209/08 , C02F2209/22 , C02F2305/06 , C12P7/625 , Y02W10/15
摘要： A process for producing PHA comprises obtaining biomass produced in the course of biologically treating a first wastewater source containing RBCOD. The biomass is to be exploited with a second wastewater source having a different RBCOD content from the first wastewater source in order to accumulate and thereby produce PHA. Before subjecting the biomass to a PHA accumulation process, the biomass PHA accumulation potential is enhanced via an acclimation process with the second wastewater source. During acclimation, the biomass is subjected to repeated feast-famine periods. During each feast period, the biomass is exposed to a fraction of the second wastewater source. The RBCOD uptake and/or biomass respiration rate is directly or indirectly measured during each feast period. The famine period is maintained for a period of time that is at least two times greater than the length of time of the proceeding feast period. After at least two feast-famine acclimation periods or after one or more measured parameters reveal an increased RBCOD relative uptake or respiration rate of the biomass during a subsequent feast period, the biomass is subjected to a PHA accumulation process using the second wastewater source.
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