公开(公告)号：US06511845B1

公开(公告)日：2003-01-28

申请号：US09618360

申请日：2000-07-18

申请人： Alan R. Davis ,  Paul P. Hung ,  Michael D. Lubeck ,  Robert J. Natuk ,  Pranab K. Chanda ,  Shridhara C. S. Murthy ,  Shaw-Guang L. Lee

发明人： Alan R. Davis ,  Paul P. Hung ,  Michael D. Lubeck ,  Robert J. Natuk ,  Pranab K. Chanda ,  Shridhara C. S. Murthy ,  Shaw-Guang L. Lee

IPC分类号： A61K3921

CPC分类号： C12N15/86 ,  A61K39/00 ,  C07K14/005 ,  C12N2710/10343 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  Y02A50/464 ,  Y02A50/472

摘要： This invention provides a method of protecting a primate from an infectious organism by stimulating the production of antibodies or cell mediated immunity to the infectious organism which comprises administering to said primate intranasally, intramuscularly, or subcutaneously, live recombinant adenoviruses in which the virion structural protein is unchanged from that in the native adenovirus from which the recombinant adenovirus is produced, and which contain the gene coding for the antigen corresponding to said antibodies or inducing said cell mediated immunity. Preferably, the infectious organism is HIV and the primate is a human.

摘要翻译： 本发明提供了通过刺激产生抗体或细胞介导的对感染性生物的免疫来保护灵长类从感染性生物体的方法，其包括向所述灵长类动物鼻内，肌内或皮下施用病毒结构蛋白质的活重组腺病毒 与从其产生重组腺病毒的天然腺病毒中不同，并且其含有编码对应于所述抗体的抗原的基因或诱导所述细胞介导的免疫。 优选地，感染性生物是HIV，灵长类是人。

公开(公告)号：US06867000B2

公开(公告)日：2005-03-15

申请号：US10169057

申请日：2000-12-07

申请人： Eric M. Mishkin ,  Robert J. Natuk ,  Michael W. Pride ,  Maninder K. Sidhu

发明人： Eric M. Mishkin ,  Robert J. Natuk ,  Michael W. Pride ,  Maninder K. Sidhu

IPC分类号： A61K39/12 ,  A61K39/245 ,  A61K39/39 ,  C12Q1/68

CPC分类号： A61K39/39 ,  A61K39/12 ,  A61K39/245 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55505 ,  A61K2039/55522 ,  A61K2039/55538 ,  A61K2039/57 ,  C12N2710/16634

摘要： A method for inducing and enhancing protective and/or therapeutic immunity in a mammal to HSV includes the steps of at least one immunization with an effective amount of a DNA vaccine composition which comprises a first nucleic acid molecule comprising a DNA sequence encoding the HSV type 1 or type 2 gD protein, and a second nucleic acid molecule comprising a DNA sequence encoding each Interleukin-12 heterodimer subunit. The method also comprises at least one subsequent immunization with an effective amount of a protein vaccine composition which comprises the HSV type 1 or type 2 gD protein; and the IL-12 heterodimer. A local anesthetic may be included in the DNA vaccine composition in an amount that forms one or more complexes with the nucleic acid molecules. When provided to the mammal in suitable effective dosages according to this protocol, the vaccine compositions used in this method produce an unexpectedly good protective and/or therapeutic immune response against HSV in an immunized mammal.

摘要翻译： 用于诱导和增强哺乳动物对HSV的保护和/或治疗免疫的方法包括以下步骤：用有效量的DNA疫苗组合物进行至少一次免疫，所述DNA疫苗组合物包含第一核酸分子，其包含编码HSV 1型的DNA序列 或2型GD蛋白，以及包含编码每个白细胞介素-12异二聚体亚基的DNA序列的第二核酸分子。 该方法还包括至少一次随后的免疫与有效量的包含HSV 1型或2型GD蛋白的蛋白质疫苗组合物; 和IL-12异源二聚体。 局部麻醉剂可以以与核酸分子形成一种或多种复合物的量包含在DNA疫苗组合物中。 当根据本方案以合适的有效剂量提供给哺乳动物时，在该方法中使用的疫苗组合物在免疫的哺乳动物中产生意想不到的良好的针对HSV的保护性和/或治疗性免疫应答。