公开(公告)号：US11987805B2

公开(公告)日：2024-05-21

申请号：US17484476

申请日：2021-09-24

申请人： Albert Einstein College of Medicine

发明人： William R. Jacobs, Jr. ,  Lawrence Leung ,  Regy Lukose ,  Anna Paula de Oliveira

IPC分类号： C12N15/01 ,  C07K14/35 ,  C12N7/00 ,  C12N9/22 ,  C12N15/09 ,  C12N15/11 ,  C12N15/90 ,  C12Q1/70

CPC分类号： C12N15/907 ,  C07K14/35 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/70 ,  C12N2310/20 ,  C12N2710/16652 ,  C12N2800/80

摘要： A genetically modified mammalian cell and genetically modified mammalian cell line comprise a recombination sequence inserted in a target locus on a chromosome of the mammalian cell genome, wherein the recombination sequence comprises Bxb1attB sequence from Mycobacterium smegmatis. A transgenic mammalian cell and transgenic mammalian cell line comprise a heterologous nucleic acid stably integrated in a target locus on a chromosome of the mammalian genome, wherein the heterologous nucleic comprises a heterologous gene configured for expression by the transgenic mammalian cell.

公开(公告)号：US20220090146A1

公开(公告)日：2022-03-24

申请号：US17484476

申请日：2021-09-24

申请人： Albert Einstein College of Medicine

发明人： William R. Jacobs, Jr. ,  Lawrence Leung ,  Regy Lukose ,  Anna Paula de Oliveira

IPC分类号： C12N15/90 ,  C07K14/35 ,  C12N7/00 ,  C12Q1/70 ,  C12N9/22 ,  C12N15/11

摘要： A genetically modified mammalian cell and genetically modified mammalian cell line comprise a recombination sequence inserted in a target locus on a chromosome of the mammalian cell genome, wherein the recombination sequence comprises Bxb1attB sequence from Mycobacterium smegmatis. A transgenic mammalian cell and transgenic mammalian cell line comprise a heterologous nucleic acid stably integrated in a target locus on a chromosome of the mammalian genome, wherein the heterologous nucleic comprises a heterologous gene configured for expression by the transgenic mammalian cell.