公开(公告)号：US20070238155A1

公开(公告)日：2007-10-11

申请号：US11487547

申请日：2006-07-13

申请人： Alexander Gusakov ,  Tatyana Salanovich ,  Alexey Antonov ,  Boris Ustinov ,  Oleg Okunev ,  Richard Burlingame ,  Mark Emalfarb ,  Marco Baez ,  Arkady Sinitsyn

发明人： Alexander Gusakov ,  Tatyana Salanovich ,  Alexey Antonov ,  Boris Ustinov ,  Oleg Okunev ,  Richard Burlingame ,  Mark Emalfarb ,  Marco Baez ,  Arkady Sinitsyn

IPC分类号： C12P19/04 ,  A01H5/00 ,  C07H21/00 ,  C07K14/00 ,  C12N1/14 ,  C12P7/24 ,  C12P7/02 ,  C12N9/14 ,  C12P1/00 ,  C12P13/04

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.运气型酶的新型酶组合物，包括CBH Ib CBH IIb，EG II，EG VI，β-葡糖苷酶和木聚糖酶II结合先前鉴定的酶CBH 1a，CBH IIa（以前描述为 Endo 43）和EG V.这些酶组合物表现出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，Douglas冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。