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公开(公告)号：US6030802A
公开(公告)日：2000-02-29
申请号：US94235
申请日：1998-06-09
CPC分类号： C12Q1/28 , C12Q1/26 , C12Q2326/00 , G01N2333/904 , G06F2207/7219 , Y10S435/962 , Y10S435/963 , Y10S435/975
摘要： The invention provides sets of liquid reagents that afford calibration stability in an enzyme based spectrophotometric assay for the measurement of lactate in patient samples. The reagent sets include a lactate oxidase, a peroxidase, a hydrogen donor, an agent that substantially prevents ascorbic acid interference, and agent that substantially prevents bilirubin interference, a coupling agent, a buffer and, optionally, a preservative. The invention further provides methods for using the liquid reagent sets.
摘要翻译： 本发明提供了一组液体试剂，其在基于酶的分光光度测定法中提供了用于测量患者样品中乳酸盐的校准稳定性。 试剂组包括乳酸氧化酶，过氧化物酶，氢供体，基本上防止抗坏血酸干扰的试剂，以及基本上防止胆红素干扰的试剂，偶联剂，缓冲剂和任选的防腐剂。 本发明还提供了使用液体试剂组的方法。
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公开(公告)号：US5804403A
公开(公告)日：1998-09-08
申请号：US791920
申请日：1997-01-31
CPC分类号： C12Q1/32
摘要： A stable aqueous NAD reagent useful in the measurement of analytes in biological fluids, particularly ethanol and lactate. For the determination of alcohol, the reagent contains alcohol dehydrogenase, pyrazole, NAD, SUTTOCIDE A and an aliphatic, zwitterionic buffer having a secondary or tertiary amine group. Preferred buffers are TAPSO, AMPSO, POPSO, TAPS and DIPSO. For the determination of lactate, the reagent contains lactate dehydrogenase, pyrazole, NAD, SUTTOCIDE A and an aliphatic, zwitterionic buffer having a secondary or tertiary amine group.
摘要翻译： 可用于测量生物液体中特别是乙醇和乳酸盐分析物的稳定的NAD水解试剂。 为了测定醇，试剂含有醇脱氢酶，吡唑，NAD，SUTTOCIDE A和具有仲胺或叔胺基团的脂肪族两性离子缓冲液。 优选的缓冲液是TAPSO，AMPSO，POPSO，TAPS和DIPSO。 为了测定乳酸盐，试剂含有乳酸脱氢酶，吡唑，NAD，SUTTOCIDE A和具有仲胺或叔胺基团的脂肪族两性离子缓冲液。
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