公开(公告)号：US09388447B2

公开(公告)日：2016-07-12

申请号：US14821574

申请日：2015-08-07

Applicant: Amgen Inc.

Inventor： Matthew I. Jerums ,  Amanda Kano ,  Henry Lin ,  Shun Luo ,  Jian Wu ,  Rebecca E. McCoy ,  Arvia E. Morris

IPC: C12N5/077 ,  C12P21/00 ,  C12N5/00

CPC classification number: C12P21/00 ,  C12N5/0037 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2510/02 ,  C12N2511/00

Abstract: The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.