公开(公告)号：US08084033B2

公开(公告)日：2011-12-27

申请号：US12169336

申请日：2008-07-08

申请人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfrey ,  David W. Buck

发明人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfrey ,  David W. Buck

IPC分类号： A61K39/00 ,  A61K39/395 ,  C07K16/00 ,  C07K17/00 ,  C12P21/08

CPC分类号： C07K14/705 ,  C07K16/28 ,  G01N33/56966 ,  Y10S530/806 ,  Y10S530/838

摘要： Compositions comprising a cell expressing an AC133 cell surface antigen and an antibody, or antigen binding fragment thereof, that specifically binds to the antigen are provided. Expression of the antigen is highly tissue specific. It is detected on a subset of hematopoietic progenitor and stem cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供了包含特异性结合抗原的表达AC133细胞表面抗原的细胞和抗体或其抗原结合片段的组合物。 抗原的表达是高度组织特异性的。 在源自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞和干细胞的子集上检测到。 由AC133识别的细胞亚群是CD34bright并且基本上包含存在于CD34 +群体中的所有CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其他造血干细胞和祖细胞标记进一步纯化。

公开(公告)号：US06455678B1

公开(公告)日：2002-09-24

申请号：US08842382

申请日：1997-04-23

申请人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfry ,  David W. Buck

发明人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfry ,  David W. Buck

IPC分类号： C07K100

CPC分类号： C07K14/705 ,  C07K16/28 ,  G01N33/56966 ,  Y10S530/806 ,  Y10S530/838

摘要： A hematopoietic progenitor cell antigen and reagents, notably antibodies, that specifically bind to the antigen are provided. Expression of the antigen is highly tissue specific. It is only detected on a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供了特异性结合抗原的造血祖细胞抗原和试剂，特别是抗体。 抗原的表达是高度组织特异性的。 仅在衍生自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞的子集上检测到。 由AC133识别的细胞亚群是CD34bright并且基本上包含存在于CD34 +群体中的所有CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其他造血干细胞和祖细胞标记进一步纯化。

公开(公告)号：US20090093052A1

公开(公告)日：2009-04-09

申请号：US12169336

申请日：2008-07-08

申请人： Amy H. Yin ,  Sheri Miraglia ,  David W. Buck

发明人： Amy H. Yin ,  Sheri Miraglia ,  David W. Buck

IPC分类号： C12N5/06 ,  C12N5/00

CPC分类号： C07K14/705 ,  C07K16/28 ,  G01N33/56966 ,  Y10S530/806 ,  Y10S530/838

摘要： A hematopoietic progenitor cell antigen and reagents, notably antibodies, that specifically bind to the antigen are provided. Expression of the antigen is highly tissue specific. It is only detected on a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供了特异性结合抗原的造血祖细胞抗原和试剂，特别是抗体。 抗原的表达是高度组织特异性的。 仅在衍生自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞的子集上检测到。 由AC133识别的细胞亚群是CD34bright并且基本上包含存在于CD34 +群体中的所有CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其他造血干细胞和祖细胞标记进一步纯化。