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1.发明授权Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme A derived compounds 有权使用磷酸酮醇酶和磷酸转乙酰酶生产乙酰辅酶A衍生化合物公开(公告)号：US09410214B2
公开(公告)日：2016-08-09
申请号：US14214062
申请日：2014-03-14
申请人： Amyris, Inc.
发明人： Kristy Michelle Hawkins , Tina Tipawan Mahatdejkul-Meadows , Adam Leon Meadows , Lauren Barbara Pickens , Anna Tai , Annie Ening Tsong
CPC分类号： C12Y401/02009 , C12N9/1029 , C12N9/16 , C12N9/88 , C12P5/007 , C12P7/54 , C12Y203/01008 , C12Y301/03021 , Y02P20/52
摘要： Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.
摘要翻译： 本文提供了用于在宿主细胞中改进乙酰辅酶A和乙酰辅酶A衍生化合物生产的组合物和方法。 在一些实施方案中，宿主细胞经遗传修饰以包含编码磷酸酮糖醇酶（PK）的异源核苷酸序列，以及将乙酰磷酸酯转化为乙酸盐的内源酶的功能性破坏。 在一些实施方案中，宿主细胞还包含编码磷酸转乙酰酶（PTA）的异源核苷酸序列。 在一些实施方案中，将乙酰磷酸酯转化为乙酸酯的酶是甘油-1-磷酸酶。 在一些实施方案中，甘油-1-磷酸酶是GPP1 / RHR2。 在一些实施方案中，甘油-1-磷酸酶是GPP2 / HOR2。 本文描述的组合物和方法为异源生产乙酰辅酶A衍生化合物（包括但不限于类异戊二烯，聚酮化合物和脂肪酸）提供了有效途径。
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2.发明申请USE OF PHOSPHOKETOLASE AND PHOSPHOTRANSACETYLASE FOR PRODUCTION OF ACETYL-COENZYME A DERIVED COMPOUNDS 有权磷酸酯酶和磷酸酯酶用于生产乙酰苯胺衍生化合物的用途公开(公告)号：US20140273144A1
公开(公告)日：2014-09-18
申请号：US14214062
申请日：2014-03-14
申请人： Amyris, Inc.
发明人： Kristy Michelle Hawkins , Tina Tipawan Mahatdejkul-Meadows , Adam Leon Meadows , Lauren Barbara Pickens , Anna Tai , Annie Ening Tsong
IPC分类号： C12P5/02
CPC分类号： C12Y401/02009 , C12N9/1029 , C12N9/16 , C12N9/88 , C12P5/007 , C12P7/54 , C12Y203/01008 , C12Y301/03021 , Y02P20/52
摘要： Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.
摘要翻译： 本文提供了用于在宿主细胞中改进乙酰辅酶A和乙酰辅酶A衍生化合物生产的组合物和方法。 在一些实施方案中，宿主细胞经遗传修饰以包含编码磷酸酮糖醇酶（PK）的异源核苷酸序列，以及将乙酰磷酸酯转化为乙酸盐的内源酶的功能性破坏。 在一些实施方案中，宿主细胞还包含编码磷酸转乙酰酶（PTA）的异源核苷酸序列。 在一些实施方案中，将乙酰磷酸酯转化为乙酸酯的酶是甘油-1-磷酸酶。 在一些实施方案中，甘油-1-磷酸酶是GPP1 / RHR2。 在一些实施方案中，甘油-1-磷酸酶是GPP2 / HOR2。 本文描述的组合物和方法为异源生产乙酰辅酶A衍生化合物（包括但不限于类异戊二烯，聚酮化合物和脂肪酸）提供了有效途径。
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