-
公开(公告)号：US20130052715A1
公开(公告)日：2013-02-28
申请号：US13641844
申请日：2011-04-20
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： The invention relates to a method for producing folded prethrombin, wherein inclusion bodies, which contain unfolded prethrombin or a derivative thereof, are solubilized in a solubilization buffer containing at least one chaotropic compound and at least one organic disulfide compound. The invention further relates to methods for producing thrombin and a-thrombin and derivatives thereof. The invention also relates to solutions that contain folded proteins, which can be produced by the methods according to the invention.
摘要翻译： 本发明涉及一种生产折叠凝血酶原的方法，其中包含未折叠凝血酶原或其衍生物的包涵体溶解于含有至少一种离液序列化合物和至少一种有机二硫化物的增溶缓冲液中。 本发明还涉及产生凝血酶和α-凝血酶及其衍生物的方法。 本发明还涉及可以通过根据本发明的方法生产的折叠蛋白质的溶液。
-
公开(公告)号：US06309861B1
公开(公告)日：2001-10-30
申请号：US09553498
申请日：2000-04-20
IPC分类号： C12P2106
CPC分类号： C12N9/6459 , C07K1/1133 , C07K2319/00 , C07K2319/02 , C12N1/20 , C12P21/02 , C12Y304/21069 , Y10S435/849 , Y10S435/877
摘要： A process produces a water-soluble, naturally folded eukaryotic polypeptide containing two or several cysteines linked by disulfide bridges. This process involves culturing prokaryotic cells, a) in which the prokaryotic cells contain an expression vector which encodes the polypeptide which contains a prokaryotic signal sequence at the N-terminus, b) under conditions under which the polypeptide is secreted into the periplasm or the medium, c) cleaving the signal sequence and isolating the polypeptide from the periplasm or the medium. In this process, the culturing is carried out in the presence of arginine or a compound of the formula I R2—CO—NR1 (I) in which R and R1 represent hydrogen or a saturated or unsaturated branched or unbranched C1-C4 alkyl chain and R2 represents hydrogen, NHR1 or a saturated or unsaturated branched or unbranched C1-C3 alkyl chain, is suitable for the recombinant production of polypeptides in prokaryotes in a high yield.
摘要翻译： 一种方法产生含有两个或几个通过二硫键连接的半胱氨酸的水溶性天然折叠的真核多肽。 该方法包括培养原核细胞，a）其中原核细胞含有编码在N末端含有原核信号序列的多肽的表达载体，b）在多肽分泌到周质或培养基中的条件下 ，c）切割信号序列并从周质或培养基中分离多肽。 在该方法中，在精氨酸或其中R和R 1表示氢或饱和或不饱和的支链或非支链C 1 -C 4烷基链的式I R 2 -CO-NR 1（I）的化合物存在下进行培养， R2代表氢，NHR1或饱和或不饱和的支链或非支链C1-C3烷基链，适合以高产率在原核生物中重组生产多肽。
-
3.发明授权Process for the production of naturally folded and secreted proteins by co-secretion of molecular chaperones 有权通过分子伴侣的共分泌生产天然折叠和分泌的蛋白质的方法公开(公告)号：US06455279B1
公开(公告)日：2002-09-24
申请号：US09618869
申请日：2000-07-19
IPC分类号： C12P2106
CPC分类号： C12N9/6459 , C07K2319/02 , C12N1/20 , C12P21/02 , C12Y304/21069
摘要： A process for the production of a naturally folded eukaryotic polypeptide containing two or several cysteines linked by disulfide bridges by a) culturing prokaryotic cells in which the said prokaryotic cells contain an expression vector which codes for the said polypeptide which contains a prokaryotic signal sequence at the N-terminus, b) secreting the polypeptide into the periplasm or the medium, c) cleaving the signal sequence and isolating the polypeptide from the periplasm or the medium, which is characterized in that a nucleic acid coding for a molecular chaperone is additionally expressed in the said prokaryotic cell and the chaperone is secreted into the periplasm, is suitable for the recombinant production of polypeptides in prokaryotes in a high yield.
摘要翻译： 一种生产包含通过二硫键连接的两个或几个半胱氨酸的天然折叠的真核生物多肽的方法，其方法是：a）培养原核细胞，其中所述原核细胞含有表达载体，所述表达载体编码所述多肽，其含有原核信号序列 N末端，b）将多肽分泌到周质或培养基中，c）切割信号序列并从周质或培养基中分离多肽，其特征在于编码分子伴侣的核酸另外表达于 所述原核细胞和分子伴侣分泌到周质中，适于以高产率重组生产原核生物中的多肽。
-
-
搜索结果
3 条记录 (耗时 0.019 秒)
