公开(公告)号：US20060071160A1

公开(公告)日：2006-04-06

申请号：US11226855

申请日：2005-09-14

申请人： Andreas Haase ,  Markus Kayser ,  Jens Hohndorf ,  Armin Holle

发明人： Andreas Haase ,  Markus Kayser ,  Jens Hohndorf ,  Armin Holle

IPC分类号： H01J49/00

CPC分类号： H01J49/164 ,  H01S3/005 ,  H01S3/08022

摘要： The invention relates to a laser system for the ionization of a sample by matrix-assisted laser desorption in mass spectrometric analysis. The invention consists in providing a laser system which generates a spatially modulated intensity distribution with intensity spots on the sample, which significantly improves the quality and the robustness of the mass spectrometric analysis compared with a spatially homogeneous intensity distribution and, in particular, improves the ionization efficiency and, for a time-of-flight mass spectrometer with axial injection, the mass resolution and the signal-to-noise ratio.

摘要翻译： 本发明涉及一种用于在质谱分析中通过基质辅助激光解吸电离样品的激光系统。 本发明提供一种激光系统，其产生具有样品上强度斑点的空间调制强度分布，与空间均匀的强度分布相比，显着提高了质谱分析的质量和鲁棒性，并且特别地提高了电离 效率，对于具有轴向注射的飞行时间质谱仪，质量分辨率和信噪比。

公开(公告)号：US20060169914A1

公开(公告)日：2006-08-03

申请号：US11314966

申请日：2005-12-21

申请人： Armin Holle ,  Jens Hohndorf ,  Andreas Haase ,  Markus Kayser

发明人： Armin Holle ,  Jens Hohndorf ,  Andreas Haase ,  Markus Kayser

IPC分类号： H01J27/24

CPC分类号： H01S3/09415 ,  H01J49/164 ,  H01S3/005 ,  H01S3/1118 ,  H01S3/115 ,  H01S3/1611 ,  H01S3/1638 ,  H01S5/041 ,  H01S5/14 ,  H01S5/183

摘要： An ion source generating ions by matrix-assisted laser desorption/ionization (MALDI) comprising a MALDI sample support and a solid-state the laser system generating a pulsed laser beam, which has a wavelength in the range between 332 and 342 nanometers and is spatially shaped in the solid-state laser system such that the spatial intensity distribution of the laser beam on the MALDI sample support exhibits more than one intensity peak.

摘要翻译： 通过基质辅助激光解吸/电离（MALDI）产生离子的离子源包括MALDI样品支持体和固态激光系统，其产生脉冲激光束，其波长在332和342纳米之间并且在空间上 在固体激光系统中成形，使得激光束在MALDI样品支架上的空间强度分布呈现多于一个强度峰值。

公开(公告)号：US20090039282A1

公开(公告)日：2009-02-12

申请号：US12177434

申请日：2008-07-22

申请人： Andreas Haase ,  Jens Hohndorf

发明人： Andreas Haase ,  Jens Hohndorf

IPC分类号： H01J27/02 ,  B01D59/44

CPC分类号： H01J49/164

摘要： Analyte ions are generated in an ion source by matrix-assisted laser desorption (MALDI) in which laser light pulses have significantly less than one nanosecond duration, focal diameters of less than twenty micrometers and energy densities such that only about one picogram of sample is desorbed per pulse of laser light and per laser spot. An unexpectedly high degree of ionization of analyte molecules is produced for selected matrix substances. Many laser spots can be generated side-by-side from a single laser light pulse for use with MALDI time-of-flight mass spectrometers. Applying pulses with a repetition rate of around 50 kilohertz and moving the sample or guiding the laser light beam so each laser light pulse impinges on a cool sample spot allows the ion source to be used with spectrometers that require a constant ion current.

摘要翻译： 分析离子通过基质辅助激光解吸（MALDI）在离子源中产生，其中激光脉冲具有显着小于一纳秒的持续时间，焦点直径小于20微米，能量密度使得仅约一个皮克的样品解吸 每个激光脉冲和每个激光点。 对于选定的基质物质产生了分析物分子意外高度的电离。 可以从单个激光脉冲并排生成许多激光点，以便与MALDI飞行时间质谱仪一起使用。 施加重复频率约为50千赫兹的脉冲，并移动样品或引导激光束，使每个激光脉冲入射到一个较冷的样品点上，使离子源与需要恒定离子电流的光谱仪一起使用。

公开(公告)号：US20060186332A1

公开(公告)日：2006-08-24

申请号：US11351350

申请日：2006-02-09

申请人： Andreas Haase ,  Markus Kayser ,  Jens Hohndorf ,  Armin Holle

发明人： Andreas Haase ,  Markus Kayser ,  Jens Hohndorf ,  Armin Holle

IPC分类号： H01J49/00

CPC分类号： H01J49/164 ,  H01J49/067

摘要： The invention relates to a laser system for the ionization of a sample by matrix-assisted laser desorption in mass spectrometric analysis. The invention consists in providing an adjustable laser system which, in one setting, generates a single intensity peak on the sample and, in another setting, a multiplicity of intensity peaks, with the half-width, intensity, spatial arrangement and/or degree of spatial modulation of the single intensity peak and/or the intensity peaks being adjustable.

摘要翻译： 本发明涉及一种用于在质谱分析中通过基质辅助激光解吸电离样品的激光系统。 本发明提供了一种可调激光系统，其在一个设置中在样品上产生单个强度峰值，并且在另一设置中产生多个强度峰值，其具有半宽度，强度，空间排列和/或程度 单个强度峰值和/或强度峰值的空间调制是可调节的。