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1.发明授权Thermostable xylanase for the selective hydrolysis of pentose-containing polysaccharides 有权用于选择性水解含糖的多糖的热稳定的木聚糖酶公开(公告)号：US08679795B2
公开(公告)日：2014-03-25
申请号：US13517344
申请日：2010-12-15
申请人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
发明人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译： 本发明涉及具有木聚糖酶活性的多肽和编码此类多肽的核酸序列。 本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及用于产生和使用多肽的方法。 木聚糖酶的一个具体应用是含有半纤维素的植物生物质的戊糖糖成分的选择性水解。 核苷酸序列可用于生产木聚糖酶或其优化的突变体。
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2.发明申请THERMOSTABLE XYLANASE FOR THE SELECTIVE HYDROLYSIS OF PENTOSE-CONTAINING POLYSACCHARIDES 有权用于选择性水解含PENTOSE的多糖的热稳定性XYLANASE公开(公告)号：US20130109062A1
公开(公告)日：2013-05-02
申请号：US13517344
申请日：2010-12-15
申请人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
发明人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译： 本发明涉及具有木聚糖酶活性的多肽和编码此类多肽的核酸序列。 本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及用于产生和使用多肽的方法。 木聚糖酶的一个具体应用是含有半纤维素的植物生物质的戊糖糖成分的选择性水解。 核苷酸序列可用于生产木聚糖酶或其优化的突变体。
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公开(公告)号：US20130123115A1
公开(公告)日：2013-05-16
申请号：US13578291
申请日：2011-02-11
申请人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
发明人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/24 , C12Y302/01091
摘要： The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.
摘要翻译： 本发明公开了具有优化的用于加工含纤维素和木质素纤维素的底物的纤维素酶。 特别地，公开了具有优选特性的纤维二糖水解酶。 本发明提供了这种酶的融合，插入，缺失和/或取代变体。 酶变体在极端pH下具有增强的热稳定性，蛋白水解稳定性，比活度和/或稳定性。 公开了编码所述酶的核酸分子，包含所述酶的组合物，制备方法以及用于纤维素加工和/或生产生物燃料的用途。
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公开(公告)号：US09458443B2
公开(公告)日：2016-10-04
申请号：US13578291
申请日：2011-02-11
申请人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
发明人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/24 , C12Y302/01091
摘要： The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.
摘要翻译： 本发明公开了具有优化的用于加工含纤维素和木质素纤维素的底物的纤维素酶。 特别地，公开了具有优选特性的纤维二糖水解酶。 本发明提供了这种酶的融合，插入，缺失和/或取代变体。 酶变体在极端pH下具有增强的热稳定性，蛋白水解稳定性，比活性和/或稳定性。 公开了编码所述酶的核酸分子，包含所述酶的组合物，制备方法以及用于纤维素加工和/或生产生物燃料的用途。
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公开(公告)号：US09593319B2
公开(公告)日：2017-03-14
申请号：US13881882
申请日：2011-10-26
CPC分类号： C12N9/2402 , C07K2319/02 , C07K2319/20 , C12N9/2437 , C12N9/248 , C12N15/52 , C12N15/62
摘要： The invention relates to temperature-stable polypeptides with β-pyranosidase activity. The polypeptide substrates include β-glucopyranosides and β-xylopyranosides. The polypeptides can be expressed alone or as fusion proteins for example in yeast or bacteria and subsequently purified. The polypeptides according to the invention can be used alone or in a mixture with other enzymes for the degradation of plant raw materials, among others for the enzymatic degradation of biomass containing lignocellulose, in particular hemicellulose and the hemicellulose component xylan. The enzymes are suitable for use in textile processing, as an additive of detergents, or in the food or feed industry.
摘要翻译： 本发明涉及具有β-吡喃糖苷酶活性的温度稳定多肽。 多肽底物包括β-吡喃葡萄糖苷和β-木兰糖苷。 多肽可以单独表达或作为融合蛋白例如在酵母或细菌中表达并随后纯化。 根据本发明的多肽可以单独使用或与其它酶的混合物一起用于植物原料的降解，其中包括用于木质纤维素，特别是半纤维素和半纤维素组分木聚糖的生物质的酶促降解。 这些酶适用于纺织加工，作为洗涤剂的添加剂，或在食品或饲料工业中。
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公开(公告)号：US20080206827A1
公开(公告)日：2008-08-28
申请号：US12076388
申请日：2008-03-18
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.
摘要翻译： 本发明涉及一种生产手性α-羟基羧酸结晶酸的方法，其通过在红球菌红霉素NCIMB 11540的存在下分别通过酶水解将氰尿酸（R）或（S）转化成α-羟基羧酸（R）或（S） 。
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7.发明授权Method for producing chiral α-hydroxycarboxylic acids by enzymatic hydrolysis of chiral cyanohydrins 失效通过手性氰醇的酶水解制备手性α-羟基羧酸的方法公开(公告)号：US07514245B2
公开(公告)日：2009-04-07
申请号：US11222020
申请日：2005-09-09
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.
摘要翻译： 本发明涉及一种生产手性α-羟基羧酸结晶酸的方法，其通过在红球菌红霉素NCIMB 11540的存在下分别通过酶水解将氰尿酸（R）或（S）转化成α-羟基羧酸（R）或（S） 。
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公开(公告)号：US07390647B2
公开(公告)日：2008-06-24
申请号：US10544103
申请日：2004-01-30
IPC分类号： C12P41/00
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.
摘要翻译： 本发明涉及一种生产手性α-羟基羧酸结晶酸的方法，其通过在红球菌红霉素NCIMB 11540的存在下分别通过酶水解将氰尿酸（R）或（S）转化成α-羟基羧酸（R）或（S） 。
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公开(公告)号：US20060199256A1
公开(公告)日：2006-09-07
申请号：US11222020
申请日：2005-09-09
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.
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公开(公告)号：US20130330784A1
公开(公告)日：2013-12-12
申请号：US13881882
申请日：2011-10-26
IPC分类号： C12N9/24
CPC分类号： C12N9/2402 , C07K2319/02 , C07K2319/20 , C12N9/2437 , C12N9/248 , C12N15/52 , C12N15/62
摘要： The invention relates to temperature-stable polypeptides with β-pyranosidase activity. The polypeptide substrates include β-glucopyranosides und β-xylopyranosides. Said polypeptides can be expressed alone or as fusion proteins for example in yeast or bacteria and subsequently purified. The polypeptides according to said invention can be used alone or in a mixture with other enzymes for the degradation of plant raw materials, among others for the enzymatic degradation of biomass containing lignocellulose, in particular hemicellulose and the hemicellulose component xylan. Said enzymes are suitable for use in textile processing, as an additive of detergents, or in the food or feed industry.
摘要翻译： 本发明涉及具有β-吡喃糖苷酶活性的温度稳定多肽。 多肽底物包括β-吡喃葡萄糖苷和β-吡喃木糖苷。 所述多肽可以单独表达或作为融合蛋白例如在酵母或细菌中表达并随后纯化。 根据本发明的多肽可以单独使用或与其它酶的混合物一起使用，用于降解植物原料，其中包括用于木质纤维素，特别是半纤维素和半纤维素组分木聚糖的生物质的酶促降解。 所述酶适用于纺织加工，作为洗涤剂的添加剂，或在食品或饲料工业中。
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