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    Basic fibroblast growth factor
    2.
    发明授权
    Basic fibroblast growth factor 失效
    碱性成纤维细胞生长因子

    公开(公告)号：US5155214A

    公开(公告)日：1992-10-13

    申请号：US462126

    申请日：1990-01-08

    申请人： Andrew J. Baird ,  Frederick S. Esch ,  Denis Gospodarowicz ,  Peter Bohlen ,  Nicholas C. Ling

    发明人： Andrew J. Baird ,  Frederick S. Esch ,  Denis Gospodarowicz ,  Peter Bohlen ,  Nicholas C. Ling

    IPC分类号： A61K38/00 ,  C07K14/50

    CPC分类号： C07K14/503 ,  A61K38/00

    摘要： Substantially pure mammalian basic fibroblast growth factors are produced. The amino acid residue sequences of bovine and human bFGF are disclosed as well as a DNA chain encoding the polypeptide of the bovine species. By appropriately inserting a synthesized DNA chain into a cloning vector and using the cloning vector to transform cells, synthetic bFGF can be obtained from transformed cell lines, both prokaryotic and eukaryotic.

    摘要翻译： 产生基本纯化的哺乳动物碱性成纤维细胞生长因子。 公开了牛和人bFGF的氨基酸残基序列以及编码牛种多肽的DNA链。 通过将合成的DNA链适当地插入到克隆载体中并使用克隆载体来转化细胞，可以从原核和真核的转化细胞系获得合成的bFGF。

    Porcine GRF
    4.
    发明授权
    Porcine GRF 失效
    猪GRF

    公开(公告)号：US4610976A

    公开(公告)日：1986-09-09

    申请号：US527292

    申请日：1983-08-29

    申请人： Peter Bohlen ,  Frederick S. Esch ,  Nicholas C. Ling ,  Paul E. Brazeau, Jr. ,  Roger C. L. Guillemin

    发明人： Peter Bohlen ,  Frederick S. Esch ,  Nicholas C. Ling ,  Paul E. Brazeau, Jr. ,  Roger C. L. Guillemin

    IPC分类号： A61K38/00 ,  C07K14/60 ,  C07K7/10 ,  A61K37/24

    CPC分类号： C07K14/60 ,  A61K38/00 ,  Y10S930/12

    摘要： The invention provides synthetic peptides which are extremely potent in stimulating the release of pituitary GH in mammals and which have the formula: H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Arg-Val-Arg-Leu-Y wherein Y is OH or NH.sub.2. These peptides or biologically active fragments thereof, or analogs thereof having well-known substitutions and, or additions, as well as nontoxic salts of any of the foregoing, may be administered therapeutically to mammals, including humans, and may be used diagnostically. The peptides are useful in stimulating the release of GH and accelerating growth in warm-blooded non-human animals, particularly pigs, and in improving aquiculture.

    摘要翻译： 本发明提供了非常有效刺激哺乳动物垂体GH释放的合成肽，其具有下式：H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys- Val-Leu-Gly-Gln-Leu- Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu -Gln-Gly-Ala-Arg-Val-Arg-Leu-Y，其中Y是OH或NH2。 这些具有众所周知的替代和/或添加的肽或其类似物或其类似物以及前述任何一种的无毒盐可以在治疗上施用于包括人在内的哺乳动物，并且可以在诊断上使用。 这些肽可用于刺激GH的释放并加速温血非人类动物，特别是猪的生长和改善水产养殖。

    Ovine GRF
    5.
    发明授权
    Ovine GRF 失效
    牛GRF

    公开(公告)号：US4605643A

    公开(公告)日：1986-08-12

    申请号：US585814

    申请日：1984-03-02

    申请人： Peter Bohlen ,  Paul E. Brazeau ,  Frederick S. Esch ,  Nicholas C. Ling ,  William B. Wehrenberg

    发明人： Peter Bohlen ,  Paul E. Brazeau ,  Frederick S. Esch ,  Nicholas C. Ling ,  William B. Wehrenberg

    IPC分类号： A61K38/00 ,  C07K14/60 ,  A61K37/43 ,  C07K7/10

    CPC分类号： C07K14/60 ,  A61K38/00 ,  Y10S930/12

    摘要： A synthetic peptide is extremely potent in stimulating the release of pituitary GH in mammals, particularly in sheep, since it is the replicate of the native (hormone) releasing factor of the sheep hypothalamus. It contains the following sequence: Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Ile-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu. This peptide or a biologically active fragment thereof, or analogs thereof having well-known substitutions and/or additions, as well as nontoxic salts of any of the foregoing, may be administered therapeutically to mammals and may be used diagnostically. The peptide is particularly useful in stimulating the release of GH so as to accelerate growth in warm-blooded non-human animals, particularly sheep, and/or to improve aquiculture.

    摘要翻译： 合成肽在刺激哺乳动物，特别是绵羊中垂体GH的释放方面是非常有效的，因为它是羊下丘脑的天然（激素）释放因子的复制物。 它含有以下序列：Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Ile-Leu-Gly-Gln-Leu-Se r-Ala-Arg-Lys-Leu -Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu。 具有众所周知的取代和/或添加的这种肽或其生物活性片段或其类似物以及前述任何一种的无毒盐可以在哺乳动物的治疗上施用，并且可以在诊断上使用。 该肽特别可用于刺激GH的释放，以加速温血非人类动物，特别是绵羊的生长和/或改善水产养殖。

    Mammalian PGRF
    6.
    发明授权
    Mammalian PGRF 失效
    哺乳动物PGRF

    公开(公告)号：US4517181A

    公开(公告)日：1985-05-14

    申请号：US418248

    申请日：1982-09-15

    申请人： Nicholas C. Ling ,  Frederick S. Esch ,  Peter Bohlen ,  Paul E. Brazeau, Jr. ,  Roger C. L. Guillemin

    发明人： Nicholas C. Ling ,  Frederick S. Esch ,  Peter Bohlen ,  Paul E. Brazeau, Jr. ,  Roger C. L. Guillemin

    IPC分类号： C07K14/575 ,  A23K1/16 ,  A61K20060101 ,  A61K38/00 ,  A61K38/04 ,  A61P5/00 ,  C07C20060101 ,  C07K14/00 ,  C07K14/60 ,  C07K14/61 ,  A61K37/02 ,  C07C103/52

    CPC分类号： C07K14/60 ,  A23K20/147 ,  A61K38/00 ,  Y10S930/12

    摘要： PGRF has been synthesized. The invention provides synthetic peptides which are extremely potent in stimulating the release of pituitary GH in mammals and which have the formula: ##STR1## wherein R is OH or NH.sub.2, R.sub.34 is Ser or Ala, R.sub.38 is Arg or Ser, R.sub.40 is Ala or Arg and R.sub.41 is Arg, Arg-Ala, Arg-Ala-Arg, Arg-Ala-Arg-Leu or des-R.sub.41. These peptides or biologically active fragments thereof, or analogs thereof having well-known substitutions and/or additions, as well as nontoxic salts of any of the foregoing, may be administered therapeutically to mammals, including humans, and may be used diagnostically.

    摘要翻译： PGRF已经合成。 本发明提供在刺激哺乳动物垂体GH释放方面非常有效的合成肽，其具有下式：H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys- Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-R34-Asn-Gln-Glu- R38-Gly-R40-R41-R其中R是OH或NH2，R34是Ser或Ala，R38是Arg或Ser，R40是Ala或Arg，R41是Arg，Arg-Ala，Arg-Ala-Arg，Arg- Ala-Arg-Leu或des-R41。 具有众所周知的取代和/或添加的这些肽或其类似物或其类似物以及前述任何一种的无毒盐可以在治疗上施用于哺乳动物，包括人，并且可以在诊断上使用。

    Bovine GRF
    7.
    发明授权
    Bovine GRF 失效
    牛GRF

    公开(公告)号：US4585756A

    公开(公告)日：1986-04-29

    申请号：US541167

    申请日：1983-10-12

    申请人： Paul E. Brazeau, Jr. ,  Peter Bohlen ,  Frederick S. Esch ,  Nicholas C. Ling ,  Roger C. L. Guillemin

    发明人： Paul E. Brazeau, Jr. ,  Peter Bohlen ,  Frederick S. Esch ,  Nicholas C. Ling ,  Roger C. L. Guillemin

    IPC分类号： A61K38/00 ,  C07K14/60 ,  A61K37/02

    CPC分类号： C07K14/60 ,  A61K38/00 ,  Y10S930/12

    摘要： The invention provides synthetic peptides which are extremely potent in stimulating the release of pituitary GH in mammals and which have the formula: H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu-Y wherein Y is OH or NH.sub.2. These peptides or biologically active fragments thereof, or analogs thereof having well-known substitutions and/or additions, as well as nontoxic salts of any of the foregoing, may be administered therapeutically to mammals, including humans, and may be used diagnostically. The peptides are useful in stimulating the release of GH so as to accelerate growth in warm-blooded non-human animals, particularly cattle, and/or to increase the production of milk in lactating cows, and also in improving aquiculture.

    摘要翻译： 本发明提供了非常有效刺激哺乳动物垂体GH释放的合成肽，其具有下式：H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys- Val-Leu-Gly-Gln-Leu- Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu -Gln-Gly-Ala-Lys-Val-Arg-Leu-Y，其中Y是OH或NH2。 具有众所周知的取代和/或添加的这些肽或其类似物或其类似物以及前述任何一种的无毒盐可以在治疗上施用于哺乳动物，包括人，并且可以在诊断上使用。 这些肽可用于刺激GH的释放，以加速温血非人类动物，特别是牛的生长，和/或增加哺乳期牛的奶的产量，以及改善水产养殖。

    Isolation of fibroblast growth factor
    8.
    发明授权
    Isolation of fibroblast growth factor 失效
    分离成纤维细胞生长因子

    公开(公告)号：US4902782A

    公开(公告)日：1990-02-20

    申请号：US268000

    申请日：1988-11-07

    申请人： Denis Gospodarowicz ,  Andrew Baird ,  Peter Bohlen

    发明人： Denis Gospodarowicz ,  Andrew Baird ,  Peter Bohlen

    IPC分类号： C07K14/50

    CPC分类号： C07K14/503 ,  Y10S530/827 ,  Y10S530/829 ,  Y10S530/837 ,  Y10S530/849

    摘要： Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.

    摘要翻译： 碱性成纤维细胞生长因子（FGF）通过使用与肝素连接的支持材料的亲和层析基本上纯化。 描述了从哺乳动物脑或哺乳动物垂体组织中提取碱性FGF的简化三步法。 盐沉淀，例如用硫酸铵，用于提供部分纯化的沉淀，然后进行离子交换层析，例如使用羧甲基Sephadex柱。 然后通过使用肝素连接的载体例如肝素 - 琼脂糖凝胶上的亲和层析分离进一步部分纯化的级分来获得基本上纯的碱性FGF级分。

    Isolation of fibroblast growth factor
    9.
    发明授权
    Isolation of fibroblast growth factor 失效

    公开(公告)号：US4785079A

    公开(公告)日：1988-11-15

    申请号：US940524

    申请日：1986-12-10

    申请人： Denis Gospodarowicz ,  Andrew Baird ,  Peter Bohlen

    发明人： Denis Gospodarowicz ,  Andrew Baird ,  Peter Bohlen

    IPC分类号： C07K14/50 ,  C07K3/28

    CPC分类号： C07K14/503

    摘要： Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.