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5 条记录 (耗时 0.035 秒)

    Error checking in blood analyzer
    1.
    发明授权
    Error checking in blood analyzer 失效
    血液分析仪检查错误

    公开(公告)号：US5590052A

    公开(公告)日：1996-12-31

    申请号：US227554

    申请日：1994-04-14

    申请人： Anne R. Kopf-Sill ,  Steven N. Buhl ,  Glenda L. Choate ,  Lloyd A. Schick ,  Robert E. Nagle ,  Jenq C. Chang ,  Daniel Bernstein ,  Wayne A. Britt

    发明人： Anne R. Kopf-Sill ,  Steven N. Buhl ,  Glenda L. Choate ,  Lloyd A. Schick ,  Robert E. Nagle ,  Jenq C. Chang ,  Daniel Bernstein ,  Wayne A. Britt

    IPC分类号： G01N21/25 ,  G01N35/00 ,  G01N35/02 ,  G01N21/17

    CPC分类号： G01N35/00594 ,  G01N21/253 ,  G01N35/025 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165

    摘要： The present invention provides various methods for detecting errors in a blood analysis system. The system includes a blood analyzer and a molded plastic rotor with a series of chambers and capillary channels through which blood is processed and distributed to cuvettes which contain lyophilized reagents. The rotor is placed in the analyzer which spins the rotor, and an optical system reads the cuvettes as light is flashed through the cuvettes. The invention provides checks for: confirming the presence of a reagent in the cuvettes before a sample is applied to the cuvettes; determining whether a fluid sample has been properly distributed to the cuvettes of the rotor; determining whether an adequate amount of fluid sample was applied to the analytical rotor; determining whether a fluid sample has been properly distributed to a cuvette of an analytical rotor; determining whether a reaction chemistry in a cuvette has reached an end point; checking the noise level and non-linearity of a reaction rate in a cuvette; determining whether an adequate amount of diluent was delivered to the cuvettes of an analytical rotor; determining dilution systematic failure when measuring different reaction chemistries in the cuvettes of the rotor; determining whether a blood sample in a cuvette is hemolyzed, lipemic, or icteric; determining the degradation of a reagent in a cuvette; determining proper light source and light detector operation; and determining proper motor function and cuvette mark detection.

    摘要翻译： 本发明提供了用于检测血液分析系统中的错误的各种方法。 该系统包括血液分析仪和具有一系列腔室和毛细通道的模制塑料转子，通过血液分析器和毛细管通道将血液加工并分配到含有冻干试剂的比色皿。 将转子放置在旋转转子的分析仪中，当光通过反应杯闪烁时，光学系统读取比色皿。 本发明提供了以下检查：在将样品施加到比色杯之前，确认试剂在试管中的存在; 确定流体样品是否已经被适当地分配到转子的比色杯中; 确定足够量的流体样品是否被施加到分析转子; 确定流体样品是否已经被适当地分配到分析转子的比色杯中; 确定比色皿中的反应化学物质是否达到终点; 检查反应杯中的反应速率的噪声水平和非线性; 确定足够量的稀释剂是否被输送到分析转子的比色杯中; 测量转子的比色杯中的不同反应化学物质时的稀释系统失效; 确定比色皿中的血液样品是否溶血，脂肪或黄疸; 确定试剂在试管中的降解; 确定适当的光源和光检测器的操作; 并确定适当的运动功能和比色皿标记检测。

    Enzyme quantitation wicking assay
    4.
    发明授权
    Enzyme quantitation wicking assay 失效
    酶定量芯吸试验

    公开(公告)号：US5188938A

    公开(公告)日：1993-02-23

    申请号：US777489

    申请日：1991-10-15

    申请人： Pyare L. Khanna ,  Glenda L. Choate

    发明人： Pyare L. Khanna ,  Glenda L. Choate

    IPC分类号： G01N33/542 ,  G01N33/558 ,  G01N33/58

    CPC分类号： G01N33/581 ,  G01N33/542 ,  G01N33/558 ,  Y10S435/97 ,  Y10S435/975

    摘要： Diagnostic assays are provided comprising complementary enzyme fragments of .beta.-galactosidase, where one of the fragments is bound to a support and the other fragment is conjugated to an immunologically cross-reactive epitope of the analyte or complementary to an analyte receptor. Binding to the receptor allows for discrimination between complexed enzyme fragment conjugate and uncomplexed enzyme fragment conjugate. The assay medium is then allowed to wick onto a support to which the complementary fragment is substantially uniformly bound in the detection region. The support is then developed, where color formation from the substrate in the detection region is used as a measure of the presence of analyte in the sample.

    摘要翻译： 提供诊断测定法，其包括β-半乳糖苷酶的互补酶片段，其中一个片段与载体结合，另一个片段与分析物的免疫交叉反应性表位缀合或与分析物受体互补。 与受体的结合允许区分复合酶片段缀合物和未复合酶片段缀合物。 然后允许测定培养基吸收到在检测区域中互补片段基本上均匀结合的载体上。 然后开发支持体，其中使用检测区域中底物的颜色形成作为样品中分析物存在的量度。

    Visual discrimination qualitative enzyme complementation assay
    5.
    发明授权
    Visual discrimination qualitative enzyme complementation assay 失效
    视觉鉴别定性酶互补测定

    公开(公告)号：US5037735A

    公开(公告)日：1991-08-06

    申请号：US210825

    申请日：1988-06-24

    申请人： Pyare L. Khanna ,  Glenda L. Choate

    发明人： Pyare L. Khanna ,  Glenda L. Choate

    IPC分类号： G01N33/543 ,  G01N33/542 ,  G01N33/558 ,  G01N33/58

    CPC分类号： G01N33/581 ,  G01N33/558

    摘要： A .beta.-galactosidase complementation assay for determining the presence of an analyte which is a member of a specific binding pair (sbp) in a sample is provided, which assay permits visual discrimination between those samples wherein the analyte is present above a predetermined, threshold concentration. The method comprises combining the sample with a hydrophobic enzyme donor- (ED-) analyte conjugate and the complementary member of the sbp to form an sbp complex-containing assay medium. A small volume of the assay medium is spotted onto an enzyme acceptor (EA) affixed to a bibulous, solid support, followed by development with enzyme substrate solution. A substantially larger area is detectable when analyte in the sample is below as compared to above a threshold concentration. The assay is particularly useful in field testing applications such as determining the presence of antibiotics in milk, toxins in water, or drugs in serum or urine. Kits facilitating the method are also provided.