公开(公告)号：US20240294978A1

公开(公告)日：2024-09-05

申请号：US18441809

申请日：2024-02-14

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay

IPC: C12Q1/6869 ,  C12Q1/00 ,  G01N27/12 ,  G01N33/487 ,  G16B30/20 ,  G16B40/10

CPC classification number: C12Q1/6869 ,  C12Q1/005 ,  G01N33/48721 ,  G16B30/20 ,  G16B40/10 ,  G01N27/125

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure provides methods of obtaining a bioelectronic signature based on current fluctuations that correspond to the activity of an enzyme-of-interest. As described herein, certain aspects of the bioelectronic signature can be used to determine the sequence of a biopolymer.

公开(公告)号：US20210340614A1

公开(公告)日：2021-11-04

申请号：US17246123

申请日：2021-04-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay

IPC: C12Q1/6869 ,  G01N27/04 ,  G01N33/483

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure relates to methods for sequencing a polynucleotide using a bioelectronic device that obtains a bioelectronic signature (e.g., current amplitude levels) of polymerase activity based on current fluctuations as complementary nucleotidepolyphosphate monomers (e.g., having distinct charges) are incorporated into the template polynucleotide.

公开(公告)号：US20200333321A1

公开(公告)日：2020-10-22

申请号：US16917474

申请日：2020-06-30

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Yanan Zhao

IPC: G01N33/487

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

公开(公告)号：US10267785B2

公开(公告)日：2019-04-23

申请号：US15922436

申请日：2018-03-15

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay ,  Peiming Zhang

IPC: G01N33/68 ,  G01N33/543 ,  G01N33/487 ,  G01N27/447 ,  G01N27/26 ,  G01N15/12 ,  C12Q1/37

Abstract: Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with the first and second volumes of fluid, and means for applying an electric potential difference between the first and second volumes of fluid. In some such embodiments, a conjugate product is provided which includes charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving the product in the first volume of fluid, the product is directed into the orifice.

公开(公告)号：US09810681B2

公开(公告)日：2017-11-07

申请号：US14851933

申请日：2015-09-11

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Shuai Chang ,  Jin He ,  Peiming Zhang ,  Shuo Huang

IPC: G01N27/00 ,  G01N33/487 ,  C12Q1/68 ,  G01N27/327

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/3275 ,  Y10T436/143333

Abstract: The invention includes compositions, devices, and methods for analyzing a polymer and/or polymer unit. The polymer may be a homo or hetero-polymer such as DNA, RNA, a polysaccharide, or a peptide. The device includes electrodes that form a tunnel gap through which the polymer can pass. The electrodes are functionalized with a reagent attached thereto, and the reagent is capable of forming a transient bond to a polymer unit. When the transient bond forms between the reagent and the unit, a detectable signal is generated and used to analyze the polymer.

公开(公告)号：US11913070B2

公开(公告)日：2024-02-27

申请号：US17184201

申请日：2021-02-24

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay

IPC: C12Q1/6869 ,  C12Q1/00 ,  G16B30/20 ,  G16B40/10 ,  G01N33/487 ,  G01N27/12

CPC classification number: C12Q1/6869 ,  C12Q1/005 ,  G01N33/48721 ,  G16B30/20 ,  G16B40/10 ,  G01N27/125

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure provides methods of obtaining a bioelectronic signature based on current fluctuations that correspond to the activity of an enzyme-of-interest. As described herein, certain aspects of the bioelectronic signature can be used to determine the sequence of a biopolymer.

公开(公告)号：US20210269869A1

公开(公告)日：2021-09-02

申请号：US17184201

申请日：2021-02-24

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay

IPC: C12Q1/6869 ,  C12Q1/00 ,  G01N33/487 ,  G16B30/20 ,  G16B40/10

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure provides methods of obtaining a bioelectronic signature based on current fluctuations that correspond to the activity of an enzyme-of-interest. As described herein, certain aspects of the bioelectronic signature can be used to determine the sequence of a biopolymer.

公开(公告)号：US20190256616A1

公开(公告)日：2019-08-22

申请号：US16155972

申请日：2018-10-10

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Peiming Zhang ,  Xu Wang ,  Jong One Im ,  Stuart Lindsay

IPC: C08B37/00 ,  G16B40/10 ,  G16B40/20 ,  G06N3/08 ,  G06N20/10

Abstract: The present disclosure provides a method for identifying and quantifying sulfated glycosaminoglycans, including for example heparin, by passing a sample through nanopores. The glycosaminoglycans sample is measured in microliter quantities, at nanomolar concentrations with detection of impurities below 0.5%, and a dynamic range over five decades of magnitude with a trained machine learning algorithm.

公开(公告)号：US10330632B2

公开(公告)日：2019-06-25

申请号：US15203383

申请日：2016-07-06

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay ,  Peiming Zhang

IPC: G01N33/487 ,  C12Q1/68 ,  G01N27/327 ,  C12Q1/6869 ,  G01N27/02

Abstract: An system for recognition of a translocating polymeric target molecule includes a device having at least one constriction that is sized to permit translocation of only a single copy of the molecule. A pair of spaced apart sensing electrodes border the constriction, which may be a nanopore. The first electrode is connected to a first affinity element and the second electrode is connected to a second affinity element. Each affinity element may connected to its corresponding electrode via one or more intermediary compounds, such as a linker molecule and/or an electrode attachment molecule. The first and second affinity elements are configured to temporarily form hydrogen bonds with first and second portions of the target molecule as the latter passes through the constriction. During translocation, the electrodes affinity elements and first and second portions of the target molecule complete an electrical circuit and allow a measurable electrical current to pass between the first and second electrodes. The time-varying nature of this electrical current, and the specific affinity elements employed, allow one to characterize the target molecule.

公开(公告)号：US10145846B2

公开(公告)日：2018-12-04

申请号：US15303960

申请日：2015-04-16

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Pei Pang

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/487

Abstract: A sensing device is provided that includes a tunnel junction created by forming a hole in a layered tunnel junction (for example). A chemically, well-defined surface may be formed by coupling affinity reagents to the electrodes, which, by these means, the surface may be configured to be selective for a particular analyte.