公开(公告)号：US20210024904A1

公开(公告)日：2021-01-28

申请号：US16756649

申请日：2017-11-06

申请人： Asymchem Life Science (Tianjin) Co., Ltd

发明人： Hao HONG ,  Gage JAMES ,  Jiangping LU ,  Xuecheng JIAO ,  Na ZHANG ,  Rui LI ,  Kejian ZHANG

IPC分类号： C12N9/10 ,  C12P13/00 ,  C12P17/12

摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.

公开(公告)号：US20210095262A1

公开(公告)日：2021-04-01

申请号：US17118169

申请日：2020-12-10

申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD. ,  ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD. ,  TIANJIN ASYMCHEM PHARMACEUTICALS CO., LTD. ,  ASYMCHEM LABORATORIES (FUXIN) CO., LTD. ,  JILIN ASYMCHEM LABORATORIES CO., LTD.

发明人： Hao HONG ,  Gage JAMES ,  Jiangping LU ,  Na ZHANG ,  Wenyan YU ,  Fang LIU ,  Yanjun LI ,  Xin HUANG ,  Juan GAO ,  Kejian ZHANG ,  Yulei MA ,  Junlu WEI

IPC分类号： C12N9/02 ,  C12N15/70 ,  C12N15/81

摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.