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公开(公告)号：US20210095262A1
公开(公告)日：2021-04-01
申请号：US17118169
申请日：2020-12-10
申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD. , ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD. , TIANJIN ASYMCHEM PHARMACEUTICALS CO., LTD. , ASYMCHEM LABORATORIES (FUXIN) CO., LTD. , JILIN ASYMCHEM LABORATORIES CO., LTD.
发明人： Hao HONG , Gage JAMES , Jiangping LU , Na ZHANG , Wenyan YU , Fang LIU , Yanjun LI , Xin HUANG , Juan GAO , Kejian ZHANG , Yulei MA , Junlu WEI
摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.
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公开(公告)号：US20230313169A1
公开(公告)日：2023-10-05
申请号：US17998916
申请日：2021-02-18
发明人： Hao HONG , Gage JAMES , Yi XIAO , Na ZHANG , Williams VYASA , Yuxia CUI , Jiadong ZHAO , Yanyan GAO
IPC分类号： C12N11/084 , C12N11/04
CPC分类号： C12N11/084 , C12N11/04
摘要： Described herein are a PVA membrane immobilized enzyme and a preparation method therefor. The PVA membrane immobilized enzyme includes a PVA porous membrane and an enzyme entrapped on the PVA porous membrane. The PVA porous membrane is a three-dimensional structured PVA porous membrane. The enzyme is any one selected from transaminase, D-lactate dehydrogenase, cyclohexanone monooxygenase, ketoreductase, alkene reductase, nitrilase, ammonia lyase, amino acid dehydrogenase, imine reductase, alcohol dehydrogenase, ammonium formate dehydrogenase, glucose 1-dehydrogenase and mutants thereof. The three-dimensional structured PVA porous membrane is used as a carrier to immobilize an enzyme in an entrapment manner. After entrapping and immobilizing the enzyme in the PVA porous membrane, the enzyme is stable, and cannot be easily leached out in the process of use. The PVA porous membrane is suitable for use in continuous flow biochemical catalysis, and has wide applicability to enzymes.
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公开(公告)号：US20220220519A1
公开(公告)日：2022-07-14
申请号：US17615262
申请日：2019-05-30
发明人： Hao HONG , Gage JAMES , Na ZHANG , Fang LIU , Junjie YAN , Ye LIU , Zujian WANG
摘要： Provided are a transaminase mutant and a method for producing a chiral amine using the same. An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by mutation occurred in an amino acid sequence as shown in SEQ ID NO: 1, and the mutation includes at least one of the following mutation sites: position 3, position 5, position 8, position 25, position 32, position 45, position 56, position 59, position 60, position 84, position 86, position 164, position 176, position 178, position 180, position 187, position 197, position 206, position 207, position 242, position 245, position 319 and position 324.
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公开(公告)号：US20210024904A1
公开(公告)日：2021-01-28
申请号：US16756649
申请日：2017-11-06
发明人： Hao HONG , Gage JAMES , Jiangping LU , Xuecheng JIAO , Na ZHANG , Rui LI , Kejian ZHANG
摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.
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公开(公告)号：US20230257720A1
公开(公告)日：2023-08-17
申请号：US18001014
申请日：2020-07-08
发明人： Hao HONG , Gage JAMES , Yi XIAO , Na ZHANG , Fang LIU , Zujian WANG , Wanming SHI , Junjie YAN , Mujiao ZHANG
CPC分类号： C12N9/0004 , C12N15/70 , C12P7/02 , C12Y101/01
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein “/” means “or.” The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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公开(公告)号：US20240010997A1
公开(公告)日：2024-01-11
申请号：US18267326
申请日：2021-04-25
发明人： Hao HONG , Gage JAMES , Na ZHANG , Xuecheng JIAO , Yulei MA , Shan CAO , Yibing CHENG , Kaihua SUN
CPC分类号： C12N9/0081 , C12N15/70 , C12P7/02 , C12P7/24
摘要： Provided are a cytochrome P450 enzyme mutant and an application thereof. The enzyme activity and anti-Markov oxidation selectivity of P450 derived from a wild-type strain of Bacillus megaterium undergo protein modification by means of directed evolution, thus improving enzyme activity and selectivity, and developing a series of P450 enzyme mutants that may be used for industrial production.
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公开(公告)号：US20210054428A1
公开(公告)日：2021-02-25
申请号：US16966528
申请日：2018-02-05
发明人： Hao HONG , Gage JAMES , Jiangping LU , Xingfu XU , Yuxia CUI , Na ZHANG , Xuewu DONG , Wenyan YU , Xin HUANG , Mingmin HAO , Yulei MA , Yibing CHENG , Jiadong ZHAO
摘要： The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.
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公开(公告)号：US20220403421A1
公开(公告)日：2022-12-22
申请号：US17774891
申请日：2019-11-07
发明人： Hao HONG , Gage JAMES , Yi HSIAO , Na ZHANG , Xuecheng JIAO , Kejian ZHANG , Yiming YANG , Xiang WANG , Yanqing ZHANG
摘要： Disclosed are a ketoreductase mutant and a method for producing a chiral alcohol. The ketoreductase mutant has an amino acid sequence obtained by the mutation of the amino acid sequence shown in SEQ ID NO: 1, and the mutation includes a mutation siteK200H. In the present disclosure, the mutant obtained by mutation takes a ketone compound as a raw material, the chiral alcohol may be efficiently produced by stereoselective reduction, and the stability is greatly improved, which is suitable for popularization and application to the industrial production of the chiral alcohol.
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公开(公告)号：US20220380817A1
公开(公告)日：2022-12-01
申请号：US17755266
申请日：2019-10-25
发明人： Hao HONG , Gage JAMES , Yi XIAO , Fang LIU , Na ZHANG , Zujian WANG , Junjie YAN , Wanming SHI , Mujiao ZHANG , Ye LIU
摘要： An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by a mutation of an amino acid sequence is shown in SEQ ID NO: 1. The mutation occurred at least one of the following mutation sites: G17V, L36P, Q40H, G69Y, H70T, L73A, V77G, V77S, V77T, A78I, Y130M, Y130V, Y130T, N132I, N132T, K141S, K142S, K142T, R143P, G144F, G144W, G144Y, E145D, E145S, E145G, K146R, L148A, L148I and the like.
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公开(公告)号：US20230295604A1
公开(公告)日：2023-09-21
申请号：US17789235
申请日：2019-12-25
发明人： Hao HONG , Gage JAMES , Yi XIAO , Na ZHANG , Vyasarayani Williams RAJASEKAR , Yuxia CUI , Jiadong ZHAO , Mingmin HAO , Yanyan GAO
CPC分类号： C12N11/091 , C12N9/0006 , C12N9/0016 , C12N9/0073 , C12N9/88 , C12N9/1096 , C12N11/06 , C12Y104/01009 , C12Y114/13022 , C12Y403/01024
摘要： Provided is an immobilized enzyme, a preparation method and use thereof. The immobilized enzyme includes an enzyme and an amino resin carrier for immobilizing the enzyme, and the enzyme is selected from any one of the following enzymes: transaminase, ketoreductase, monooxygenase, ammonia-lyase, ene reductase, imine reductase, amino acid dehydrogenase, and nitrilase. The amino resin carrier is an amino resin carrier modified by a cross-linking agent, and the cross-linking agent is a cross-linking agent treated by a polymer. By means of modifying the amino resin carrier with the cross-linking agent treated by the polymer, the enzyme immobilized on the amino resin carrier may easily form a network cross-linking, such that the immobilization effect of the enzyme is more stable, thereby the recycling efficiency of the enzyme is improved.
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