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公开(公告)号：US20210095262A1
公开(公告)日：2021-04-01
申请号：US17118169
申请日：2020-12-10
申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD. , ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD. , TIANJIN ASYMCHEM PHARMACEUTICALS CO., LTD. , ASYMCHEM LABORATORIES (FUXIN) CO., LTD. , JILIN ASYMCHEM LABORATORIES CO., LTD.
发明人： Hao HONG , Gage JAMES , Jiangping LU , Na ZHANG , Wenyan YU , Fang LIU , Yanjun LI , Xin HUANG , Juan GAO , Kejian ZHANG , Yulei MA , Junlu WEI
摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.
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公开(公告)号：US20210024904A1
公开(公告)日：2021-01-28
申请号：US16756649
申请日：2017-11-06
发明人： Hao HONG , Gage JAMES , Jiangping LU , Xuecheng JIAO , Na ZHANG , Rui LI , Kejian ZHANG
摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.
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公开(公告)号：US20200239895A1
公开(公告)日：2020-07-30
申请号：US16652291
申请日：2017-11-15
发明人： Hao HONG , Gage JAMES , Jiangping LU , Xingfu XU , Wenyan YU , Xin HUANG , Yulei MA , Yibing CHENG
摘要： A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.
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公开(公告)号：US20220380280A1
公开(公告)日：2022-12-01
申请号：US17642937
申请日：2019-09-17
发明人： Hao HONG , Jiangping LU , Xichun FENG , Xin ZHANG , Bo YAN
摘要： The disclosure discloses a method for an addition reaction of acetylene and a ketone compound. The method includes the following steps: S1, providing a continuous reaction device, wherein the continuous reaction device includes a plurality of bubble tubular reactors being connected with each other through connecting tubes; feeding a raw material solution containing the ketone compound and alkali into the plurality of bubble tubular reactors, and S3, under normal pressure, pumping acetylene from the bottom of the first bubble tubular reactor for the addition reaction. By applying the technical solution of the invention, acetylene reacts with the ketone compound in the plurality of bubble tubular reactors arranged in series, which can ensure the sufficient gas-liquid contact time, and thus making full use of the acetylene gas, improving the utilization rate thereof, effectively reduing the amount of acetylene, reducing costs, and further improving the safety.
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公开(公告)号：US20220251034A1
公开(公告)日：2022-08-11
申请号：US17629840
申请日：2019-07-25
发明人： Hao HONG , Jiangping LU , Jian TAO , Yan ZHANG , Fan WANG , Gonzalez MIGUEL
IPC分类号： C07C277/06
摘要： The present disclosure provides a preparation method for metformin hydrochloride. The preparation method includes: microwave heating raw materials containing dicyandiamide and dimethylamine hydrochloride, and reacting the two at 100° C. to 160° C. to obtain a product system containing metformin hydrochloride. Microwave heating is adopted to heat the dicyandiamide and the dimethylamine hydrochloride. Compared with a conventional heating mode that requires heat to be gradually transferred from the outside to the inside, microwave heating can directly heat each part inside the reactant, which can make the internal temperature of the reactant more uniform, thereby reducing the generation of impurities.
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公开(公告)号：US20210054428A1
公开(公告)日：2021-02-25
申请号：US16966528
申请日：2018-02-05
发明人： Hao HONG , Gage JAMES , Jiangping LU , Xingfu XU , Yuxia CUI , Na ZHANG , Xuewu DONG , Wenyan YU , Xin HUANG , Mingmin HAO , Yulei MA , Yibing CHENG , Jiadong ZHAO
摘要： The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.
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公开(公告)号：US20220297080A1
公开(公告)日：2022-09-22
申请号：US17642194
申请日：2019-09-12
发明人： Hao HONG , Jiangping LU , Xichun FENG , Xin ZHANG , Bo YAN
摘要： The present disclosure provides a device for continuously preparing 2,6-dihydroxybenzaldehyde and use thereof. The device includes a first continuous reaction unit for hydroxy protection reaction, a second continuous reaction unit for lithiation and hydroformylation, and a third continuous reaction unit for deprotection reaction that are connected in series. The third continuous reaction unit includes: a first columnar continuous reactor, connected to the second continuous reaction unit and used for deprotection of the lithiated hydroformylated product while performing liquid separation to obtain an organic phase containing 2,6-dihydroxybenzaldehyde and an aqueous phase. When the device is applied in the preparation of 2,6-dihydroxybenzaldehyde, reaction time is shortened and the intermediate purification treatment is no longer required. Therefore, compared with batch process, the present disclosure can greatly save equipment cost and post-processing cost, and greatly improve the production efficiency, more beneficial to the industrial scale-up production of 2,6-dihydroxybenzaldehyde.
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公开(公告)号：US20220380273A1
公开(公告)日：2022-12-01
申请号：US17620268
申请日：2019-06-18
发明人： Hao HONG , Enxuan ZHANG , Jiangping LU , Fuliang WEI , Sihang YANG
IPC分类号： C07C1/28
摘要： Disclosed is a method for continuously synthesizing a propellane compound. The method includes the following steps: using 1,1-dibromo-2,2-bis(chloromethyl)cyclopropane or a derivative thereof as a raw material to form a ring with a lithium metal agent by a continuous reaction, so as to synthesize the propellane compound. A technical scheme of the present disclosure is applied, and a continuous reaction device is used.
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公开(公告)号：US20150299153A1
公开(公告)日：2015-10-22
申请号：US14351337
申请日：2012-10-25
发明人： Hao HONG , Pingzhong HUANG , Jiangping LU , Xin ZHANG , Xiaowen GUO , Xingfang SUN , Jian TAO
IPC分类号： C07D307/08
CPC分类号： C07D307/08 , C07D307/06
摘要： The disclosure claims a continuous processing method of 2-Methyltetrahydrofuran (2-MeTHF), including the steps as follows: introducing gasification furfural and hydrogen into a first reaction zone and processing a first catalytic hydrogenation reaction; introducing the gas which is output from the first reaction zone to a second reaction zone to implement a secondary catalytic hydrogenation reaction; and condensing the gas output from the second reaction zone to obtain the 2-MeTHF; wherein, the first reaction zone is filled with a catalyst which is used for aldehyde group reduction, and the second reaction zone is filled with the catalyst for aromatic saturation hydrogenation. By adopting the low-toxicity catalyst which is cheap and easy to get, the high-purity 2-MeTHF can be produced by implementing the gas-phase continuous reaction by the furfural under low pressure or ambient pressure, the traditional process which has high pressure, high investment and high risk can be changed.
摘要翻译： 本发明要求2-甲基四氢呋喃（2-MeTHF）的连续处理方法，其包括以下步骤：将糠醛和氢气气化引入第一反应区并处理第一催化氢化反应; 将从第一反应区输出的气体引入第二反应区以实施二次催化氢化反应; 并冷凝来自第二反应区的气体输出以获得2-MeTHF; 其中，第一反应区填充有用于醛基还原的催化剂，第二反应区填充有用于芳族饱和氢化的催化剂。 通过采用便宜而易于获得的低毒催化剂，可以通过在低压或环境压力下通过糠醛实现气相连续反应来生产高纯度2-MeTHF，传统方法具有高压 投资高，风险高可以改变。
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公开(公告)号：US20220259122A1
公开(公告)日：2022-08-18
申请号：US17618577
申请日：2019-06-11
发明人： Hao HONG , Jiangping LU , Enxuan ZHANG , Zhiqing LIU , Tao ZHANG
IPC分类号： C07C29/147 , C07B41/02
摘要： Disclosed are a borohydride reduction stabilizing system and a method for reducing an ester to an alcohol. The borohydride reduction stabilizing system includes: a borohydride reducing agent and a stabilizing agent for stabilizing the borohydride reducing agent. The borohydride reducing agent is sodium borohydride or potassium borohydride. The stabilizing agent is an alkali metal salt of an alcohol. By adding the alkali metal salt of the alcohol, such as sodium alkoxide or potassium alkoxide, on the basis of an existing sodium/potassium borohydride reducing agent, the sodium/potassium borohydride reducing agent may be kept stable without being decomposed under the condition of increased temperature, so that on the one hand, the reducing activity is maintained in a relatively high state, and the condition of excessive use is reduced, and on the other hand, the generation of hydrogen is reduced, and the process risks are reduced.
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