公开(公告)号：US20040058391A1

公开(公告)日：2004-03-25

申请号：US10671341

申请日：2003-09-25

申请人： Aventis Pharmaceuticals Inc.

发明人： Stephen A. Baum

IPC分类号： G01N033/53 ,  C12M001/34

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/0031 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00322 ,  B01J2219/00461 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/00522 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00668 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2300/0819 ,  B01L2300/0829 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  G01N33/543

摘要： A three-dimensional (3D) array of solid-phase supports is adapted to provide parallel synthesis of a library of molecules with 3D diversity. Individual locations in the 3D array may be assigned to selected molecules in the library such that molecules may be synthesized at and retrieved from such locations. Also, the supports include aperture walls in stacked plates; the supports may be suspended within stacked plate apertures; the 3D array include discrete supports arranged in columns in one or more wells; the supports include tube inner walls or be suspended in tubes, the tubes being secured in stacked, two-dimensional (2D) frameworks; or the supports include beads contained in porous enclosures having non-porous side walls and being secured in stacked, 2D frameworks. A support transfer device enables transfer of solid-phase supports used in a 3D array. Such apparatus includes: a rack of rods sized to be inserted through supports and a mechanism to prevent supports from coming off the rack; tubes connected to a vacuum manifold to suction supports one Z plane at a time; or a transfer block having recesses to receive one or more support and at least one gate withholding supports from passing through the gate when in a closed position. A method of 3D synthesis includes: a) functionalizing solid-phase supports; b) placing supports in a 3D array; and c) performing parallel synthesis with 3D diversity. At least one unique R1 group member may be assigned to each Z plane.

摘要翻译： 三维（3D）固相支架阵列适用于提供具有3D多样性的分子库的并行合成。 3D阵列中的各个位置可以分配给文库中的选定分子，使得分子可以在这些位置合成并从这些位置检索。 此外，支撑件包括堆叠板中的孔壁; 支撑件可以悬挂在堆叠的板孔内; 3D阵列包括以一个或多个孔中的列布置的离散支撑件; 支撑件包括管内壁或悬挂在管中，管被固定在堆叠的二维（2D）框架中; 或者支撑体包括包含在具有无孔侧壁并且被固定在堆叠的2D框架中的多孔封壳中的珠子。 支撑转移装置能够传送在3D阵列中使用的固相支撑。 这种装置包括：尺寸适于插入通过支撑件的杆状杆状物和防止支架从架子上脱落的机构; 连接到真空歧管以抽吸的管一次支撑一个Z平面; 或具有用于接收一个或多个支撑件的凹部的传递块和当处于关闭位置时至少一个闸门预扣支撑件不经过闸门。 一种3D合成方法包括：a）将固相载体官能化; b）将支撑放置在3D阵列中; 和c）使用3D分集进行并行合成。 可以将至少一个唯一的R1组成员分配给每个Z平面。