公开(公告)号：US12297440B2

公开(公告)日：2025-05-13

申请号：US17609966

申请日：2020-05-06

Applicant: BASF SE

Inventor： Frank Meulewaeter ,  Shirong Zhang ,  Christophe Liseron-Monfils

IPC: C12N15/82

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.

公开(公告)号：US20230203515A1

公开(公告)日：2023-06-29

申请号：US17641478

申请日：2020-09-10

Applicant: BASF SE

Inventor： Frank Meulewaeter ,  Christophe Liseron-Monfils

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.

公开(公告)号：US20230148071A1

公开(公告)日：2023-05-11

申请号：US17781904

申请日：2020-11-30

Applicant: BASF SE

Inventor： Frank Meulewaeter ,  Christophe Liseron-Monfils

IPC: C12N15/82 ,  C12N15/10

CPC classification number: C12N15/8216 ,  C12N15/1072 ,  C12N15/1065 ,  C12N15/8213

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.

公开(公告)号：US20220220495A1

公开(公告)日：2022-07-14

申请号：US17609966

申请日：2020-05-06

Applicant: BASF SE

Inventor： Frank Meulewaeter ,  Shirong Zhang ,  Christophe Liseron-Monfils

IPC: C12N15/82

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.

公开(公告)号：US20190153458A1

公开(公告)日：2019-05-23

申请号：US16178882

申请日：2018-11-02

Applicant: BASF SE

Inventor： Frank Meulewaeter ,  Bartel Vanholme

IPC: C12N15/82 ,  C12N9/10 ,  A01H5/10 ,  D06P3/66 ,  A01H5/00

Abstract: Methods and means are provided to produce positively charged oligosaccharides in the plant cell wall by introducing into said plant cell a Nodulation C protein fused to a heterologous Golgi signal anchor sequence.

公开(公告)号：US10647965B2

公开(公告)日：2020-05-12

申请号：US16118956

申请日：2018-08-31

Applicant: BASF SE ,  THE UNIVERSITY COURT OF THE UNIVERSITY OF EDINBURGH

Inventor： Frank Meulewaeter ,  Ilse Van Den Brande ,  Stephen C. Fry ,  Kyle E. Mohler ,  Lenka Frankova ,  Tom J. Simmons ,  Claire Holland ,  Andrew Hudson

IPC: C12N9/10 ,  C08L1/02 ,  D06M16/00 ,  C08B1/00 ,  C08B37/00 ,  C08H8/00 ,  D06M15/03 ,  C12N15/82 ,  C12P19/04 ,  C12P19/18

Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.

公开(公告)号：US20190085349A1

公开(公告)日：2019-03-21

申请号：US16194614

申请日：2018-12-07

Applicant: BASF SE

Inventor： Frank Meulewaeter

IPC: C12N15/82 ,  C12N9/10 ,  D02G3/02

Abstract: An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase from E. coli which is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore, methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.

公开(公告)号：US20190002849A1

公开(公告)日：2019-01-03

申请号：US16118956

申请日：2018-08-31

Applicant: BASF SE ,  The University Court of the University of Edinburgh

Inventor： Frank Meulewaeter ,  Ilse Van Den Brande ,  Stephen C. Fry ,  Kyle E. Mohler ,  Lenka Frankova ,  Tom J. Simmons ,  Claire Holland ,  Andrew Hudson

IPC: C12N9/10 ,  C12P19/18 ,  C12P19/04 ,  D06M15/03 ,  C12N15/82 ,  C08H8/00 ,  C08B1/00 ,  D06M16/00 ,  C08L1/02 ,  C08B37/00

Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.