公开(公告)号：US20220340865A1

公开(公告)日：2022-10-27

申请号：US17620786

申请日：2020-06-29

Applicant: BASF SE

Inventor： Thomas Kaeding ,  Kerstin Hage ,  Rodolfo Ham-Zhu

IPC: C12N1/20

Abstract: The present invention is directed to a method of preparing a fermentation medium comprising the steps of a) mixing components of a fermentation medium comprising a complex nutrient source in water, wherein the complex nutrient source is in an amount of 0.5-30% w/v of the fermentation medium; b) adjusting the pH of the fermentation medium obtained in step a) to a pH between pH 3.0 and pH 6.7; c) subjecting the fermentation medium subsequently to a heat treatment and thereafter adjusting the pH to a pH value suitable for cell cultivation. The present invention is further directed to a method a fermentation process comprising cultivation of the cells in the fermentation medium of the invention and the production of a compound of interest. The present invention is further directed to a method for reducing color formation and viscosity during fermentation medium preparation and during the fermentation process.