公开(公告)号：US12198329B2

公开(公告)日：2025-01-14

申请号：US17103414

申请日：2020-11-24

Applicant: BERKELEY LIGHTS, INC.

Inventor： John A. Tenney ,  Thomas M. Vetterli ,  Hansohl E. Kim

IPC: G06T7/00 ,  G06T7/11 ,  G06T7/174 ,  G06T7/30

Abstract: Methods are provided for the automated detection, characterization, and selection of micro-objects in a microfluidic device. In addition, methods are provided for grouping detected micro-objects into subgroups that share the same characteristics and, optionally, repositioning micro-objects in a selected sub-population within the microfluidic device. For example, micro-objects in a selected sub-population can be moved into sequestration pens. The methods also provide for visual displays of the micro-object characteristics, such as two- or three-dimensional graphs, and for user-based definition and/or selection of sub-populations of the detected micro-objects. In addition, non-transitory computer-readable medium in which a program is stored and systems for carrying out any of the disclosed methods are provided.

公开(公告)号：US20210349075A1

公开(公告)日：2021-11-11

申请号：US17241865

申请日：2021-04-27

Applicant: BERKELEY LIGHTS, INC.

Inventor： Yelena BRONEVETSKY ,  Annamaria MOCCIARO ,  Guido K. STADLER ,  Peter J. BEEMILLER ,  Natalie C. MARKS ,  Duane SMITH ,  Vincent Haw Tien PAI ,  Jason M. MCEWEN ,  Amanda L. GOODSELL ,  John A. TENNEY ,  Thomas M. VETTERLI ,  Hansohl E. Kim

IPC: G01N33/50 ,  B01L3/00

Abstract: Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

公开(公告)号：US11170200B2

公开(公告)日：2021-11-09

申请号：US16428792

申请日：2019-05-31

Applicant: Berkeley Lights, Inc.

Inventor： Hansohl E. Kim ,  John A. Tenney ,  Joshua F. Slocum

IPC: G06K9/00 ,  G06T7/246 ,  G06K9/62 ,  G06T5/20 ,  G06T7/73 ,  G06K9/74 ,  G06T1/00

Abstract: Methods are provided for the automated detection and/or counting of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.