公开(公告)号：US20240279724A1

公开(公告)日：2024-08-22

申请号：US18570440

申请日：2021-06-16

Applicant: BGI SHENZHEN

Inventor： Ji Wang ,  Tao Tan ,  Lin Zhou ,  Ou Wang ,  Wenwei Zhang ,  Ao Chen

IPC: C12Q1/6844 ,  C12Q1/34 ,  C12Q1/527 ,  C40B50/06

CPC classification number: C12Q1/6844 ,  C12Q1/34 ,  C12Q1/527 ,  C40B50/06

Abstract: Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.