公开(公告)号：US20230056313A1

公开(公告)日：2023-02-23

申请号：US18048671

申请日：2022-10-21

Applicant: BGI SHENZHEN

Inventor： Zhaohui Wang ,  Handong Li ,  Yuan Li ,  Wenwei Zhang ,  Ao Chen

IPC: C12Q1/6874 ,  H01L27/146 ,  B01L3/00

Abstract: Provided is a sequencing chip. The sequencing chip includes: a chip main body, nucleic acids, and a phosphonic acid polymer film. The chip main body includes a plurality of chip particles arranged in a same layer, the chip particles are obtained by cutting a chip matrix along cutting lines of a wafer layer, and the chip matrix includes: the wafer layer having the cutting lines uniformly distributed thereon; a first silicon oxide layer made of silicon oxide and formed on an upper surface of the wafer layer; and a transition metal oxide layer made of a transition metal oxide and formed on an upper surface of the first silicon oxide layer. The nucleic acids are fixed on the transition metal oxide layer; and the phosphonic acid polymer film is made of a polyphosphonic acid polymer and formed on an upper surface of the transition metal oxide layer.

公开(公告)号：US20240279724A1

公开(公告)日：2024-08-22

申请号：US18570440

申请日：2021-06-16

Applicant: BGI SHENZHEN

Inventor： Ji Wang ,  Tao Tan ,  Lin Zhou ,  Ou Wang ,  Wenwei Zhang ,  Ao Chen

IPC: C12Q1/6844 ,  C12Q1/34 ,  C12Q1/527 ,  C40B50/06

CPC classification number: C12Q1/6844 ,  C12Q1/34 ,  C12Q1/527 ,  C40B50/06

Abstract: Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.

公开(公告)号：US11649489B2

公开(公告)日：2023-05-16

申请号：US16757719

申请日：2018-10-12

Applicant: BGI SHENZHEN

Inventor： Erkai Liu ,  Wenwei Zhang ,  Ao Chen ,  Chongjun Xu

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12Q1/6876

Abstract: Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3′ end, a polymerase, a reagent 1 for excising the blocking group attached to the 3′ end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.