公开(公告)号：US20240392265A1

公开(公告)日：2024-11-28

申请号：US18564400

申请日：2021-09-08

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu ,  Zhuo Chen

IPC: C12N9/12 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided are a thermostable B-family DNA polymerase mutant and an application thereof. The thermostable B-family DNA polymerase mutant is provided to mutate amino acid residues in at least two of three sites of group A, i.e., 408, 409, and 410 in an amino acid sequence of thermostable B-family DNA polymerases to obtain proteins having DNA polymerase activity. A-group sites (408/409/410), B-group sites (451/485), C-group sites (389/383/384), D-group sites (589/67/680), E-group sites (491/493/494/497), and F-group sites (474/478/486/480/484) of several B-family DNA polymerases are analyzed to design the mutant.