公开(公告)号：US20240392263A1

公开(公告)日：2024-11-28

申请号：US18032755

申请日：2020-10-21

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Zi Wang ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.