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公开(公告)号:US20140220577A1
公开(公告)日:2014-08-07
申请号:US13924353
申请日:2013-06-21
Applicant: BIO-RAD LABORATORIES, INC.
Inventor: YAN WANG , PETER VANDER HORN , LEI XI
IPC: C12Q1/68
CPC classification number: C12N15/10 , C07K14/195 , C07K2319/00 , C07K2319/80 , C12N9/1252 , C12N15/62 , C12P19/34 , C12Q1/686 , C12Y207/07007
Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
Abstract translation: 本发明提供使用改进的核酸聚合酶的产生来测序和进行聚合酶反应的方法。 改进是以提高聚合酶的持续性的方式将序列非特异性核酸结合结构域与酶的融合。