公开(公告)号：US20230043703A1

公开(公告)日：2023-02-09

申请号：US17813942

申请日：2022-07-21

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG ,  STEVEN TAKASHI OKINO ,  JASON MA

IPC: C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/686

Abstract: The subject invention pertains to the detection and differentiation of genetic variations by nucleic acid amplification. The invention provides methods of detecting one or more genetic variations in a nucleic acid that are in close proximity simultaneously. The invention further provides primer and probe oligonucleotides and methods of using said primers and probes in assays to detect genetic variants of concern of SARS-CoV-2. The methods of the invention detect genetic variants of other pathogens, including influenza, or genetic variants involved in inheritable diseases or cancer.

公开(公告)号：US20140220577A1

公开(公告)日：2014-08-07

申请号：US13924353

申请日：2013-06-21

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG ,  PETER VANDER HORN ,  LEI XI

IPC: C12Q1/68

CPC classification number: C12N15/10 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N15/62 ,  C12P19/34 ,  C12Q1/686 ,  C12Y207/07007

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

Abstract translation: 本发明提供使用改进的核酸聚合酶的产生来测序和进行聚合酶反应的方法。 改进是以提高聚合酶的持续性的方式将序列非特异性核酸结合结构域与酶的融合。

公开(公告)号：US20140038237A1

公开(公告)日：2014-02-06

申请号：US14058044

申请日：2013-10-18

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG

IPC: C12P19/34 ,  C07K14/00

CPC classification number: C12N9/1276 ,  C07K14/00 ,  C07K14/001 ,  C07K19/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y207/07049 ,  Y02P20/52

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

Abstract translation: 本发明提供了改进的新型核酸修饰酶的产生。 改进是以提高酶结合和催化修饰核酸的能力的方式将序列非特异性核酸结合结构域与酶的融合。