公开(公告)号：US12188931B2

公开(公告)日：2025-01-07

申请号：US17562055

申请日：2021-12-27

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Michelle Delanoy ,  John Flanagan ,  Audrey Arjomandi ,  Ravi Kaul ,  Steven R. Binder

IPC: C07K14/20 ,  A61K39/02 ,  G01N33/569

Abstract: The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

公开(公告)号：US20130274125A1

公开(公告)日：2013-10-17

申请号：US13801275

申请日：2013-03-13

Applicant: BIO-RAD LABORATORIES INC.

Inventor： Steven R. Binder ,  Roger P. Walker ,  Michelle L. Delanoy

IPC: G01N33/68

CPC classification number: G01N33/6854 ,  G01N33/564 ,  G01N2800/102

Abstract: Rheumatoid arthritis and other autoimmune diseases are diagnosed by multiplex assays for antibodies to a panel of antigens that includes cyclic citrullinated peptide and at least five members of a list that includes BRAF1 506-525, BRAF2 656-675, Vimentin (protein) citrullinated, Vimentin 415-433 cit cyclic, Vimentin 58-77 cit3 cyclic, Clusterin 231-250 cit sm1 cyclic, Fibrinogen A 556-575 cit sm cyclic, Fibrinogen A 616-635 cit sm cyclic, Histones2A H2A/a 1-20 cit sm2 cyclic, Filaggrin 48-65 cit2v1 cyclic, BRAF (catalytic domain from v raf murine sarcoma viral oncogene homologue B1, amino acids 416-766).

Abstract translation: 类风湿性关节炎和其他自身免疫性疾病通过多种测定法诊断，用于抗一组抗原的抗体，包括环瓜氨酸肽和至少五个列表的成员，其中包括BRAF1 506-525，BRAF2 656-675，波形蛋白（蛋白质）瓜氨酸化，波形蛋白 波形蛋白58-77 cit3环状，Clusterin 231-250 cit sm1环状，纤维蛋白原A 556-575 cit sm cyclic，纤维蛋白原A 616-635 cit sm cyclic，Histones2A H2A / a 1-20 cit sm2 cyclic， 丝胶蛋白48-65 cit2v1环状，BRAF（来自v raf鼠肉瘤病毒致癌基因同系物B1的催化结构域，氨基酸416-766）。

公开(公告)号：US11209431B2

公开(公告)日：2021-12-28

申请号：US15766525

申请日：2016-10-06

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Michelle Delanoy ,  John Flanagan ,  Audrey Arjomandi ,  Ravi Kaul ,  Steven R. Binder

IPC: A61K39/02 ,  C07K14/10 ,  G01N33/569 ,  C07K14/20

Abstract: The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

公开(公告)号：US20130196350A1

公开(公告)日：2013-08-01

申请号：US13755234

申请日：2013-01-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steven R. Binder ,  Michelle Delanoy ,  Audrey Arjomandi ,  John Flanagan

IPC: G01N33/68 ,  G01N33/74

CPC classification number: G01N33/6893 ,  G01N33/57449 ,  G01N33/6854 ,  G01N33/74

Abstract: Improved ovarian cancer assays are provided.

Abstract translation: 提供改进的卵巢癌检测。