公开(公告)号：US12188931B2

公开(公告)日：2025-01-07

申请号：US17562055

申请日：2021-12-27

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Michelle Delanoy ,  John Flanagan ,  Audrey Arjomandi ,  Ravi Kaul ,  Steven R. Binder

IPC: C07K14/20 ,  A61K39/02 ,  G01N33/569

Abstract: The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

公开(公告)号：US20170370931A1

公开(公告)日：2017-12-28

申请号：US15701272

申请日：2017-09-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/571 ,  G01N33/543 ,  G01N33/92

CPC classification number: G01N33/571 ,  G01N33/543 ,  G01N33/54313 ,  G01N33/92 ,  G01N2333/20 ,  G01N2405/04 ,  G01N2469/20

Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.

公开(公告)号：US20190265240A1

公开(公告)日：2019-08-29

申请号：US16405588

申请日：2019-05-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/571 ,  G01N33/92 ,  G01N33/543

Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.

公开(公告)号：US20180172687A1

公开(公告)日：2018-06-21

申请号：US15897040

申请日：2018-02-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/576 ,  G01N33/543

CPC classification number: G01N33/5761 ,  G01N33/54326

Abstract: Provided herein are multiplex assays for determining whether an individual is or has been infected with Hepatitis B virus, and the stage of infection or resolution. In addition, the multiplex system can discriminate between vaccinated subjects and subjects susceptible to Hepatitis B infection.

公开(公告)号：US09927439B2

公开(公告)日：2018-03-27

申请号：US14261525

申请日：2014-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: C12Q1/68 ,  C12Q1/70 ,  A61K39/00 ,  A61K39/29 ,  C07K14/005 ,  G01N33/576 ,  G01N33/543

CPC classification number: G01N33/5761 ,  G01N33/54326

Abstract: Provided herein are multiplex assays for determining whether an individual is or has been infected with Hepatitis B virus, and the stage of infection or resolution. In addition, the multiplex system can discriminate between vaccinated subjects and subjects susceptible to Hepatitis B infection.

公开(公告)号：US11209431B2

公开(公告)日：2021-12-28

申请号：US15766525

申请日：2016-10-06

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Michelle Delanoy ,  John Flanagan ,  Audrey Arjomandi ,  Ravi Kaul ,  Steven R. Binder

IPC: A61K39/02 ,  C07K14/10 ,  G01N33/569 ,  C07K14/20

Abstract: The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

公开(公告)号：US10942182B2

公开(公告)日：2021-03-09

申请号：US16832608

申请日：2020-03-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/571 ,  G01N33/543 ,  G01N33/92

Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.

公开(公告)号：US20140323332A1

公开(公告)日：2014-10-30

申请号：US14261525

申请日：2014-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/576

CPC classification number: G01N33/5761 ,  G01N33/54326

Abstract: Provided herein are multiplex assays for determining whether an individual is or has been infected with Hepatitis B virus, and the stage of infection or resolution. In addition, the multiplex system can discriminate between vaccinated subjects and subjects susceptible to Hepatitis B infection.

Abstract translation: 本文提供用于确定个体是否已经感染乙型肝炎病毒的多重测定，以及感染或分辨的阶段。 此外，多重系统可以区分接种的受试者和易感乙型肝炎感染的受试者。

公开(公告)号：US20240248086A1

公开(公告)日：2024-07-25

申请号：US18437816

申请日：2024-02-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/571 ,  G01N33/543 ,  G01N33/92

CPC classification number: G01N33/571 ,  G01N33/543 ,  G01N33/54313 ,  G01N33/92 ,  G01N2333/20 ,  G01N2405/04 ,  G01N2469/20

Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.

公开(公告)号：US11913954B2

公开(公告)日：2024-02-27

申请号：US17170577

申请日：2021-02-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/53 ,  G01N33/571 ,  G01N33/543 ,  G01N33/92

CPC classification number: G01N33/571 ,  G01N33/543 ,  G01N33/54313 ,  G01N33/92 ,  G01N2333/20 ,  G01N2405/04 ,  G01N2469/20

Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.