公开(公告)号：US10961518B2

公开(公告)日：2021-03-30

申请号：US16248442

申请日：2019-01-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiangdong Meng ,  Man Cheng

IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6876 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/80

Abstract: Mutant Type-A DNA polymerases having increased resistance to one or more polymerization activity inhibitors are provided.

公开(公告)号：US09752177B2

公开(公告)日：2017-09-05

申请号：US14256861

申请日：2014-04-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yanhong Kong ,  Steven T. Okino ,  Xiangdong Meng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q2521/125 ,  C12Q2521/301 ,  C12Q2527/156

Abstract: Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described.

公开(公告)号：US10287620B2

公开(公告)日：2019-05-14

申请号：US15581401

申请日：2017-04-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man Cheng ,  Xiangdong Meng ,  Jan Zur Megede

IPC: C12N15/62 ,  C12Q1/48

Abstract: Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.

公开(公告)号：US20160208312A9

公开(公告)日：2016-07-21

申请号：US14256861

申请日：2014-04-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yanhong Kong ,  Steven T. Okino ,  Xiangdong Meng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q2521/125 ,  C12Q2521/301 ,  C12Q2527/156

Abstract: Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described.

公开(公告)号：US20140220586A1

公开(公告)日：2014-08-07

申请号：US14256861

申请日：2014-04-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yanhong Kong ,  Steven T. Okino ,  Xiangdong Meng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q2521/125 ,  C12Q2521/301 ,  C12Q2527/156

Abstract: Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described.

Abstract translation: 描述使用透化细胞过滤小核酸和使用过滤检测基因组DNA可及性的方法。

公开(公告)号：US11597920B2

公开(公告)日：2023-03-07

申请号：US16911421

申请日：2020-06-25

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Xiangdong Meng ,  Xiaosong Gong ,  Cindy C T Wan

IPC: C12N9/12 ,  C12N9/96 ,  C12N15/11

Abstract: The subject application pertains to compositions and methods for enhancing reverse transcriptase (RT) activity and/or reducing the inhibition of RT by inhibitors, such as formalin, tannic acid and/or heparin. In some embodiments, RT inhibition is reduced by the addition of potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188, or any combination thereof to a reaction mixture comprising a polymerase. In other embodiments, RT is enhanced through the addition of a polyvinyl sulfonic acid sodium salt (PVSA) to a reaction mixture. The subject application also provides oligonucleotide primers for use in the reverse transcription of target sequences and its enhancement. These primers have high GC content or low GC content. Methods of using a RT inhibition reducer or a RT enhancer in a composition with an RNA template and RT improves RT yield, RT sensitivity, or RT tolerance to various chemicals are also provided.