公开(公告)号：US20150353911A1

公开(公告)日：2015-12-10

申请号：US14406160

申请日：2013-06-07

Applicant: Biogen Idec MA Inc.

Inventor： Joe Salas ,  Elena Kistanova ,  Vu Phong Hong ,  Adam R. Mezo ,  Robert T. Peters

IPC: C12N9/64 ,  A61K38/48 ,  A61K47/48

CPC classification number: C12N9/6432 ,  A61K38/36 ,  A61K38/4846 ,  A61K47/62 ,  C12N9/6437 ,  C12Y304/21006 ,  C12Y304/21021

Abstract: The invention provides chimeric clotting factors comprising an activatable clotting factor and an enhancer moiety. The activatable clotting factor allows the chimeric clotting factor to be activated at the site of coagulation. The enhancer moiety can additionally improve procoagulation activities of the chimeric clotting factors. The chimeric clotting factors can further be improved by fusion to a half-life extender, which improves a pharmacokinetics property of the chimeric clotting factor. The invention also includes methods of making and methods of using these chimeric clotting factors.

Abstract translation: 本发明提供了包含可激活的凝血因子和增强子部分的嵌合凝血因子。 可激活的凝血因子允许嵌合凝血因子在凝血部位被活化。 增强子部分可另外改善嵌合凝血因子的促凝活性。 通过与半衰期延长剂的融合进一步改善嵌合凝血因子，这提高了嵌合凝血因子的药代动力学性质。 本发明还包括制备方法和使用这些嵌合凝血因子的方法。

公开(公告)号：US20150252345A1

公开(公告)日：2015-09-10

申请号：US14430848

申请日：2013-09-25

Applicant: BIOGEN IDEC MA INC.

Inventor： Glenn Pierce ,  Samantha Truex ,  Robert T. Peters ,  Haiyan Jiang ,  Mark Brader

IPC: C12N9/64

CPC classification number: C12N9/644 ,  A61K38/00 ,  C07K14/745 ,  C07K16/36 ,  C07K2317/76 ,  C07K2319/30 ,  C12Y304/21022

Abstract: The present invention provides methods of administering long-acting Factor IX; methods of administering long-acting, chimeric and hybrid polypeptides comprising Factor IX; and methods of producing such chimeric and hybrid polypeptides using cells.

Abstract translation: 本发明提供了施用长效因子IX的方法; 施用包含因子IX的长效，嵌合和杂合多肽的方法; 以及使用细胞产生这种嵌合和杂交多肽的方法。

公开(公告)号：US20150023959A1

公开(公告)日：2015-01-22

申请号：US14371948

申请日：2013-01-12

Applicant: BIOGEN IDEC MA INC.

Inventor： Ekta Seth Chhabra ,  Tongyao Liu ,  Robert T. Peters ,  Haiyan Jiang

IPC: C07K14/755 ,  C07K16/00

CPC classification number: C07K14/755 ,  A61K38/37 ,  A61K47/62 ,  C07K16/00 ,  C07K2317/94 ,  C07K2319/00 ,  C07K2319/31

Abstract: The present invention provides a VWF fragment comprising the D′ domain and D3 domain of VWF, a chimeric protein comprising the VWF fragment and a heterologous moiety, or a chimeric protein comprising the VWF fragment and a FVIII protein and methods of using the same. A polypeptide chain comprising a VWF fragment of the invention binds to or is associated with a polypeptide chain comprising a FVIII protein and the polypeptide chain comprising the VWF fragment can prevent or inhibit binding of endogenous VWF to the FVIII protein. By preventing or inhibiting binding of endogenous VWF to the FVIII, which is a half-life limiting factor for FVIII, the VWF fragment can induce extension of half-life of the FVIII protein. The invention also includes nucleotides, vectors, host cells, methods of using the VWF fragment, or the chimeric proteins.

Abstract translation: 本发明提供了包含VWF的D'结构域和D3结构域的VWF片段，包含VWF片段和异源部分的嵌合蛋白质，或包含VWF片段和FVIII蛋白质的嵌合蛋白质及其使用方法。 包含本发明的VWF片段的多肽链与包含FVIII蛋白的多肽链结合或相关联，并且包含VWF片段的多肽链可以预防或抑制内源性VWF与FVIII蛋白的结合。 通过预防或抑制内源性VWF与作为FVIII的半衰期限制因子的FVIII的结合，VWF片段可诱导FVIII蛋白的半衰期延长。 本发明还包括核苷酸，载体，宿主细胞，使用VWF片段或嵌合蛋白的方法。