公开(公告)号：US20210131946A1

公开(公告)日：2021-05-06

申请号：US16479112

申请日：2018-01-18

申请人： BIOMILLENIA SAS

发明人： Guansheng Du ,  Dirk Löffert ,  Eric Shiue

IPC分类号： G01N15/14 ,  B01L3/00

摘要： The invention relates to a microfluidic system in which the droplets flow through an off-chip delay line in a linear sequential order. This ensures that all the droplets are incubated for the same amount of time and under the same conditions as they flow through the delay line. The invention further relates to the use of this microfluidic system for high throughput screening methods or in vitro evolution methods.

公开(公告)号：US20190382711A1

公开(公告)日：2019-12-19

申请号：US16480564

申请日：2018-01-26

申请人： BIOMILLENIA SAS

发明人： Aleksander Dajkovic ,  Dirk Löffert

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12Q1/04 ,  C12N1/02

摘要： In a first aspect, the present invention relates to a method for detecting and/or selecting a microorganism with a desired trait comprising the steps of (a) providing a composition comprising two or more microorganisms, (b) subjecting said two or more microorganisms to one of the following reactions leading to a change in the genetic composition in at least one microorganism, (i) natural transformation, (ii) transduction by phage, (iii) conjugation; (c) encapsulating each microorganism in a single microfluidic droplet together with an indicator molecule capable of detecting the presence of the desired trait, (d) incubating the encapsulated microorganism together with the indicator molecule under conditions that allow the detection of the desired trait, (e) sorting said droplets in a microfluidic system into at least (i) one group where the desired trait is not detectable and (ii) one group where the desired trait is detectable if present. In a second aspect, the present invention relates to a microorganism created by a method according to the first aspect of the present invention and its relative embodiments. In a third aspect, the present invention also encompasses a microfluidic kit comprising a microfluidic chip suitable for performing the method according to the first aspect and its relative embodiments and a collection reservoir for collecting single microfluidic droplets encapsulating the microorganism with the desired trait as defined in the second aspect and its relative embodiment. A variation of the method of the first aspect in which microorganisms not carrying a desired trait are brought in contact with nucleic acids carrying the trait is also disclosed.

公开(公告)号：US11597963B2

公开(公告)日：2023-03-07

申请号：US16762733

申请日：2018-11-09

申请人： BIOMILLENIA SAS

发明人： Dirk Löffert ,  Eric Shiue ,  Aleksander Dajkovic ,  Guansheng Du

IPC分类号： C12Q1/00 ,  C12Q1/18 ,  C12Q1/04

摘要： The invention relates to a method for the identification of a first microorganism potentially secreting an effector compound, said first microorganism thereby having either i. an inhibitory effect on the cell division activity of a second microorganism or ii. an enhancing effect on the cell division activity of a second microorganism, the method comprising: a. providing a cell from a first microorganism which potentially produces an effector compound of interest and a cell from a second detector microorganism; b. introducing both cells into a microdroplet for incubation; c. introducing the microdroplet into a microfluidic system; d. analyzing in said microfluidic system the cell of the second microorganism for the exhibition of an enhanced growth effect or the exhibition of an inhibited growth effect stemming from said effector compound. The invention also relates to a microorganism or effector compound identified by the method according to the invention.

公开(公告)号：US20200263225A1

公开(公告)日：2020-08-20

申请号：US16762733

申请日：2018-11-09

申请人： BIOMILLENIA SAS

发明人： Dirk Löffert ,  Eric Shiue ,  Aleksander Dajkovic ,  Guansheng Du

IPC分类号： C12Q1/18 ,  C12Q1/04

摘要： The invention relates to a method for the identification of a first microorganism potentially secreting an effector compound, said first microorganism thereby having either i. an inhibitory effect on the cell division activity of a second microorganism or ii. an enhancing effect on the cell division activity of a second microorganism, the method comprising: a. providing a cell from a first microorganism which potentially produces an effector compound of interest and a cell from a second detector microorganism; b. introducing both cells into a microdroplet for incubation; c. introducing the microdroplet into a microfluidic system; d. analyzing in said microfluidic system the cell of the second microorganism for the exhibition of an enhanced growth effect or the exhibition of an inhibited growth effect stemming from said effector compound. The invention also relates to a microorganism or effector compound identified by the method according to the invention.