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公开(公告)号：US20210208045A9
公开(公告)日：2021-07-08
申请号：US16621328
申请日：2018-06-11
申请人： Biomillenia SAS
发明人： Aleksander Dajkovic
摘要： The invention relates to a microfluidic method for measuring viscosity in a micro droplet in a microfluidic system, comprising the steps of i) introducing a fluorescent molecule into a micro droplet otherwise comprising a fluid, ii) in the microfluidic system, exciting the fluorescent molecule in said micro droplet by applying light to the micro droplet, iii) measuring the resulting fluorescence emitted from the micro droplet thereby determining the viscosity of the fluid in the micro droplet. The invention also relates to method of screening for microorganisms or cells that produce viscosity-modulating compounds with desired properties. Finally, the invention also relates to the use of fluorescent molecules for measuring the viscosity of a fluid in a micro droplet in a microfluidic system.
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公开(公告)号：US20220409686A1
公开(公告)日：2022-12-29
申请号：US17773736
申请日：2020-10-28
申请人： BIOMILLENIA SAS
发明人： Aleksander Dajkovic , Dirk Loeffert
IPC分类号： A61K36/064 , A61K35/74 , A61P31/10 , A61P17/08
摘要： The invention relates to a pharmaceutical composition comprising (i) a live strain from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp, (ii) an attenuated strain from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp or, (iii) a medically active substance isolated from a strain from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp.
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公开(公告)号：US20190382711A1
公开(公告)日：2019-12-19
申请号：US16480564
申请日：2018-01-26
申请人： BIOMILLENIA SAS
发明人： Aleksander Dajkovic , Dirk Löffert
摘要： In a first aspect, the present invention relates to a method for detecting and/or selecting a microorganism with a desired trait comprising the steps of (a) providing a composition comprising two or more microorganisms, (b) subjecting said two or more microorganisms to one of the following reactions leading to a change in the genetic composition in at least one microorganism, (i) natural transformation, (ii) transduction by phage, (iii) conjugation; (c) encapsulating each microorganism in a single microfluidic droplet together with an indicator molecule capable of detecting the presence of the desired trait, (d) incubating the encapsulated microorganism together with the indicator molecule under conditions that allow the detection of the desired trait, (e) sorting said droplets in a microfluidic system into at least (i) one group where the desired trait is not detectable and (ii) one group where the desired trait is detectable if present. In a second aspect, the present invention relates to a microorganism created by a method according to the first aspect of the present invention and its relative embodiments. In a third aspect, the present invention also encompasses a microfluidic kit comprising a microfluidic chip suitable for performing the method according to the first aspect and its relative embodiments and a collection reservoir for collecting single microfluidic droplets encapsulating the microorganism with the desired trait as defined in the second aspect and its relative embodiment. A variation of the method of the first aspect in which microorganisms not carrying a desired trait are brought in contact with nucleic acids carrying the trait is also disclosed.
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公开(公告)号：US20230405057A1
公开(公告)日：2023-12-21
申请号：US17781276
申请日：2020-10-28
申请人： BIOMILLENIA SAS
发明人： Aleksander Dajkovic , Dirk Loeffert
IPC分类号： A61K35/74 , A61K36/064 , A61P31/10
CPC分类号： A61K35/74 , A61P31/10 , A61K36/064
摘要： The invention also relates to a microbial strain selected from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp for use as a medicament, (i) a cellular extract from a strain selected from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp for use in treating inflammatory bowel disease, (ii) the supernatant from the cell culture from a strain selected from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp for use in treating inflammatory bowel disease or, (iii) a medically active molecule or a medically active substance obtained from a strain selected from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp for use in treating inflammatory bowel disease.
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公开(公告)号：US20220370515A1
公开(公告)日：2022-11-24
申请号：US17773403
申请日：2020-10-28
申请人： BIOMILLENIA SAS
发明人： Dirk Loeffert , Aleksander Dajkovic
IPC分类号： A61K35/74 , A23L33/135 , A23L33/14 , A61K8/9728 , A61K8/99
摘要： The invention relates to a pharmaceutical composition comprising (i) a live strain from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp, (ii) an attenuated strain from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp or, (iii) a medically active substance isolated from a strain from the genus of Kazachstania spp, Acetobacter spp. and/or Stenotrophomonas spp. These may be used to treat pancreatic cancer.
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公开(公告)号：US20200003770A1
公开(公告)日：2020-01-02
申请号：US16490804
申请日：2018-03-02
申请人： Biomillenia SAS
IPC分类号： G01N33/543 , C12N5/071 , C12Q1/02
摘要： Cultivation and/or test-system comprising at least two cultivation spaces combined in a microparticle, wherein the sperically shaped microparticle comprises, (i) a first cultivation space in the center core of said spherically shaped microparticle, (ii) a second cultivation space in the wall surrounding the core of said microparticle, (iii) wherein the wall surrounding the core allows for the exchange of molecules as, salts, nutrients, peptides, chemicals and other compounds in order for the cells in the first cultivation space to interact with the cells in second cultivation space and vice versa. In the test system, the cells are co-cultivated and the microparticles are then selected based on the phenotype of the cells in the first or second cultivation space.
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公开(公告)号：US20230340557A1
公开(公告)日：2023-10-26
申请号：US18016581
申请日：2021-07-12
申请人： BIOMILLENIA SAS
发明人： Aleksander Dajkovic , Guansheng Du , Dirk Loeffert
CPC分类号： C12Q1/025 , C12Q1/20 , B01L3/502784 , B01L7/52 , C12M23/16 , C12M41/36 , B01L2300/0829 , B01L2300/18 , B01L2200/0647
摘要： The present invention uses microfluidics droplets for studying the growth dynamics of communities of bacteria in the presence of various perturbations such as various chemicals or other microorganisms and to establish microbial assemblies model which is representative of a microbiota of interest and to examine their growth dynamics after perturbations such as with an array of chemicals or other microorganisms. In particular, the present invention refers to a method and apparatus for assessing the effect of a chemical or biological substance on the growth of microorganisms.
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公开(公告)号：US20230227920A1
公开(公告)日：2023-07-20
申请号：US18009969
申请日：2021-06-14
申请人： Biomillenia SAS
摘要： In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e. dispensing said at least one double-layer microfluidic droplet into a culture medium based on the viability of the cell, f. incubating said culture medium, thereby obtaining said library. In a second aspect, the present invention relates to a system comprising: a. a first microfluidic chip for producing a plurality of single-layer microfluidic droplets wherein at least one single-layer microfluidic droplet comprises at least one single cell, b. a first microfluidic device for collecting said plurality of single-layer microfluidic droplets, c. a second device for adding a sensing compound into said at least one single-layer microfluidic droplet comprising at least one single cell, d. a second microfluidic chip for producing a double-layer microfluidic droplet, and e. a dispensing unit. In a third aspect, the present invention relates to the use of the method according to the first aspect of the present invention in a system according to the second aspect of the present invention.
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公开(公告)号：US11597963B2
公开(公告)日：2023-03-07
申请号：US16762733
申请日：2018-11-09
申请人： BIOMILLENIA SAS
发明人： Dirk Löffert , Eric Shiue , Aleksander Dajkovic , Guansheng Du
摘要： The invention relates to a method for the identification of a first microorganism potentially secreting an effector compound, said first microorganism thereby having either i. an inhibitory effect on the cell division activity of a second microorganism or ii. an enhancing effect on the cell division activity of a second microorganism, the method comprising: a. providing a cell from a first microorganism which potentially produces an effector compound of interest and a cell from a second detector microorganism; b. introducing both cells into a microdroplet for incubation; c. introducing the microdroplet into a microfluidic system; d. analyzing in said microfluidic system the cell of the second microorganism for the exhibition of an enhanced growth effect or the exhibition of an inhibited growth effect stemming from said effector compound. The invention also relates to a microorganism or effector compound identified by the method according to the invention.
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公开(公告)号：US11448645B2
公开(公告)日：2022-09-20
申请号：US16490804
申请日：2018-03-02
申请人： Biomillenia SAS
IPC分类号： G01N33/543 , C12N5/071 , C12Q1/02
摘要： Cultivation and/or test-system comprising at least two cultivation spaces combined in a microparticle, wherein the sperically shaped microparticle comprises, (i) a first cultivation space in the center core of said spherically shaped microparticle, (ii) a second cultivation space in the wall surrounding the core of said microparticle, (iii) wherein the wall surrounding the core allows for the exchange of molecules as, salts, nutrients, peptides, chemicals and other compounds in order for the cells in the first cultivation space to interact with the cells in second cultivation space and vice versa. In the test system, the cells are co-cultivated and the microparticles are then selected based on the phenotype of the cells in the first or second cultivation space.
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