公开(公告)号：US10544435B2

公开(公告)日：2020-01-28

申请号：US15556737

申请日：2016-03-10

Applicant: BIOPETROLIA AB

Inventor： Jiufu Qin ,  Anastasia Krivoruchko ,  Florian David ,  Bo Jiang ,  Jens Nielsen

IPC: C12P13/10 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N9/88 ,  C12N13/00 ,  C12N15/52 ,  C12P13/00

Abstract: The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

公开(公告)号：US20180105850A1

公开(公告)日：2018-04-19

申请号：US15556737

申请日：2016-03-10

Applicant: BIOPETROLIA AB

Inventor： Jiufu Qin ,  Anastasia Krivoruchko ,  Florian David ,  Bo Jiang ,  Jens Nielsen

IPC: C12P13/10 ,  C12N9/10 ,  C12N9/78 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12P13/00

CPC classification number: C12P13/10 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/78 ,  C12N9/88 ,  C12N15/52 ,  C12P13/001 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y206/01011 ,  C12Y207/02008 ,  C12Y305/03001 ,  C12Y401/01017 ,  C12Y403/02001

Abstract: The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.