公开(公告)号：US20230192778A1

公开(公告)日：2023-06-22

申请号：US16630203

申请日：2018-07-12

申请人： BIOSYNTIA APS

发明人： Hans Jasper GENEE ,  Anne Pihl BALI ,  Nils MYLING-PETERSEN

IPC分类号： C07K14/245 ,  C12N15/70 ,  C12N15/77 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

CPC分类号： C07K14/245 ,  C12N15/70 ,  C12N15/77 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93

摘要： The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.

公开(公告)号：US20220127311A1

公开(公告)日：2022-04-28

申请号：US17423457

申请日：2020-01-15

申请人： BIOSYNTIA APS

发明人： Hans Jasper GENEE ,  Carlos G. ACEVE-DO-ROCHA ,  Anne Pihl BALI ,  Lasse Holm LAURIDSEN ,  Luisa GRONENBERG ,  Nils MYLING-PETERSEN

IPC分类号： C07K14/245 ,  C12N15/70 ,  C12N9/10 ,  C12N9/88 ,  C12N9/02 ,  C12N9/90 ,  C12N9/00 ,  C12N9/16 ,  C12N9/24

摘要： The invention relates to a genetically modified prokaryotic cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) and one or more transgenes or upregulated endogenous genes encoding iron-sulfur (Fe—S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements, electron transfer, radical or non-redox reactions, sulfur donation or perform regulatory functions. The prokaryotic cells are characterized by enhanced activity of these iron-sulfur (Fe—S) cluster polypeptides, enhancing their respective functional capacity, and facilitating enhanced yields of compounds in free and protein-bound forms, including heme, hemoproteins, tetrapyrroles, B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids, pyrroloquinoline quinone, ammonia, indigo, or their precursors, whose biosynthesis depends on their activity. The invention further relates to a method for producing said compounds or their precursors using the genetically modified prokaryotic cell of the invention, and the use of the genetically modified prokaryotic cell.