公开(公告)号：US12054760B2

公开(公告)日：2024-08-06

申请号：US17755191

申请日：2019-12-17

申请人： BLOOMAGE BIOTECHNOLOGY CORPORATION LIMITED ,  JIANGNAN UNIVERSITY

发明人： Zhen Kang ,  Jian Chen ,  Litao Hu ,  Guocheng Du ,  Yang Wang ,  Jianghua Li ,  Jialian Li ,  Tianmeng Zhang

IPC分类号： C12P19/04 ,  C12N1/20 ,  C12N9/10 ,  C12N9/26 ,  C12N15/77 ,  C12R1/15

CPC分类号： C12P19/04 ,  C12N1/205 ,  C12N9/1051 ,  C12N9/2474 ,  C12N15/77 ,  C12Y204/01212 ,  C12Y302/01035 ,  C12N2800/101 ,  C12R2001/15

摘要： The invention discloses a recombinant Corynebacterium glutamicum for efficient synthesis of highly pure hyaluronic acid and oligosaccharides thereof, belonging to the technical field of bioengineering. The recombinant Corynebacterium glutamicum constructed in the present invention can produce hyaluronic acid with a yield up to 40 g/L, and a crude product purity of 95%. Addition of exogenous hyaluronic acid hydrolase and optimization of the fermentation conditions results in hyaluronic acid oligosaccharides with specific molecular weight, and can further improve the yield of hyaluronic acid to 72 g/L. The invention lays a solid foundation for the efficient synthesis of highly pure hyaluronic acid by microorganisms, and the constructed recombinant Corynebacterium glutamicum is suitable for industrial production and application.