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    Method of thawing cryopreserved cells
    1.
    发明授权
    Method of thawing cryopreserved cells 失效
    解冻冷冻保存细胞的方法

    公开(公告)号：US5895745A

    公开(公告)日：1999-04-20

    申请号：US719769

    申请日：1996-09-25

    申请人： Barbara A. Chandler ,  Kermit M. Borland ,  Shawn P. Cain ,  Claudy J-P. Mullon

    发明人： Barbara A. Chandler ,  Kermit M. Borland ,  Shawn P. Cain ,  Claudy J-P. Mullon

    IPC分类号： C12N5/06 ,  A01N1/02 ,  C12N5/00 ,  G01N31/00

    CPC分类号： A01N1/0284 ,  A01N1/0221 ,  Y10T436/108331

    摘要： The invention features a method of processing cryopreserved cells by thawing and equilibrating the cells at warm temperatures (e.g., between 30.degree. C. and 43.degree. C). Either the cell suspension in the cryoprotective medium is thawed to a temperature between 35.degree. C. and 43.degree. C. or the cryoprotective medium is equilibrated with a culture medium at a temperature between 35.degree. C. and 43.degree. C., or both steps are carried out at the warm temperatures. By thawing and equilibrating the cryopreserved cells at warm temperatures, the viability, (especially after 3 hours of culture), and metabolic activity (i.e., diazepam metabolism) of the cells can be improved over traditional cold cell processing (i.e., at temperatures of between 2.degree. C. and 8.degree. C.).

    摘要翻译： 本发明的特征在于通过在温暖的温度（例如30℃至43℃）下解冻和平衡细胞来处理冷冻保存的细胞的方法。 将冷冻保护培养基中的细胞悬浮液解冻至35℃至43℃之间的温度，或将冷冻保护培养基在培养基中在35℃至43℃之间的温度下平衡，或两个步骤 在温暖的温度下进行。 通过在温暖的温度下融化和平衡冷冻保存的细胞，可以比传统的冷细胞处理（即，在两者之间的温度）之间改善细胞的存活力（特别是培养3小时）和代谢活性（即，地西泮代谢） 2℃和8℃）。

    High flow technique for harvesting mammalian cells
    2.
    发明授权
    High flow technique for harvesting mammalian cells 失效
    用于收获哺乳动物细胞的高流量技术

    公开(公告)号：US5912163A

    公开(公告)日：1999-06-15

    申请号：US953541

    申请日：1997-10-20

    申请人： Kermit M. Borland ,  Barbara A. Chandler ,  Dianna Hunt Picton ,  Shawn P. Cain ,  Deborah Deane ,  Claudy J.P. Mullon

    发明人： Kermit M. Borland ,  Barbara A. Chandler ,  Dianna Hunt Picton ,  Shawn P. Cain ,  Deborah Deane ,  Claudy J.P. Mullon

    IPC分类号： A01N1/02 ,  C07G15/00

    CPC分类号： A01N1/0242 ,  A01N1/021 ,  A01N1/0247 ,  C12M45/09

    摘要： The invention is based in part on the observation that significant portions of porcine livers appear to remain intact after perfusion by standard methods, suggesting that the perfusion procedures employed do not result in complete enzymatic digestion. Recovery of cells is therefore substantially lower than would be possible if the organs were thoroughly digested. It is found that increased perfusion flow rate, occlusion of at least one major blood vessel leading out of the organ, increased enzymatic digestion time, and vigorous tissue dissociation techniques can be combined to afford a uniquely high yield of viable cells.

    摘要翻译： 本发明部分基于观察到，通过标准方法灌注后，猪肝脏的显着部分似乎保持完整，这表明所用的灌注程序不会导致完全的酶消化。 因此，如果器官被彻底消化，则细胞的恢复显着低于可能的。 发现增加的灌注流速，至少一个从器官出来的主要血管的阻塞增加了酶消化时间，并且可以组合有力的组织解离技术以提供独特的高产量的活细胞。