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    Methods for Increasing Expression of Genes In a Fungal Cell
    1.
    发明申请
    Methods for Increasing Expression of Genes In a Fungal Cell 审中-公开
    增加真菌细胞中基因表达的方法

    公开(公告)号：US20090317866A1

    公开(公告)日：2009-12-24

    申请号：US12373715

    申请日：2007-07-13

    申请人： Barbara Cherry ,  Debbie Yaver ,  Mads Bjornvad

    发明人： Barbara Cherry ,  Debbie Yaver ,  Mads Bjornvad

    IPC分类号： C12P21/02 ,  C07K2/00 ,  C12N15/11 ,  C12N15/81 ,  C12N1/15

    CPC分类号： C12N15/81 ,  C07K14/395

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide comprising a nucleic acid sequence encoding the polypeptide operably linked to a copper-inducible promoter sequence comprising a copper-responsive upstream activation sequence activated by a copper-dependent trans-acting transcription factor and a second polynucleotide comprising one or more (several) additional copper-responsive upstream activation sequences operably linked upstream to the promoter sequence, wherein the promoter sequence is foreign to the nucleic acid sequence encoding the polypeptide and the copper-responsive upstream activation sequences are responsible for copper-induced transcription of the promoter sequence, and a third polynucleotide comprising at least one copy of a gene encoding the copper-dependent trans-acting transcription factor; and (b) isolating the polypeptide from the cultivation medium.

    摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含第一多核苷酸，其包含可操作地编码多肽的核酸序列 与包含由铜依赖性反式作用转录因子活化的铜响应上游激活序列的铜诱导型启动子序列连接，第二多核苷酸包含一个或多个（几个）另外的铜响应性上游激活序列，其可操作地连接到上游 启动子序列，其中所述启动子序列对于编码所述多肽的核酸序列是外来的，并且所述铜响应性上游激活序列负责所述启动子序列的铜诱导的转录，以及第三多核苷酸，其包含至少一个编码 铜依赖的反作用转录 n因素 和（b）从培养基中分离多肽。