公开(公告)号：US20120034623A1

公开(公告)日：2012-02-09

申请号：US13263997

申请日：2010-04-14

申请人： Bas Hulsken ,  Stein Kuiper ,  Sjoerd Stallinga ,  Bo Joakim Isaksson ,  Mark W.G. Ponjee ,  Bart E.G.J. Van Meerbergen ,  Zeynep S. Unay

发明人： Bas Hulsken ,  Stein Kuiper ,  Sjoerd Stallinga ,  Bo Joakim Isaksson ,  Mark W.G. Ponjee ,  Bart E.G.J. Van Meerbergen ,  Zeynep S. Unay

IPC分类号： G01N33/566 ,  C12M1/34 ,  C12Q1/02

CPC分类号： G01N1/4077 ,  G01N1/4005 ,  G01N15/0625 ,  G01N15/0631

摘要： A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S1) the microobjects by removing, in a total time T1, a volume V1 of the sample fluid from the upconcentrate sample microobjects; immersing (S2) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S3), in a total time T3, a volume V3 of the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S4) an image of the microobjects accumulated on the filter; wherein the throughput V1/T1 of the step of up-concentrating (S1) is greater than the throughput V1/T1, of the step of filtering (S3). The filter may be a second filter, and the step of up-concentrating (S1) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.

摘要翻译： 提出了一种分析含有机微物体样品液的方法。 该方法包括以下步骤：通过在总时间T1中去除来自上升浓度样品微物体的样品流体的体积V1来上升（S1）微观对象; 将微量物体浸入（S2）到转移流体中，或将微量物体留在样品流体的剩余部分中，然后将样品流体的剩余部分提供转移流体; 在总时间T3中过滤（S3）通过过滤器传送流体的体积V3，从而将微物体累积在过滤器上; 以及生成（S4）累积在所述过滤器上的所述微物体的图像; 其特征在于，上述浓缩步骤（S1）的吞吐量V1 / T1大于滤波步骤的吞吐量V1 / T1（S3）。 过滤器可以是第二过滤器，并且上集中（S1）的步骤可以包括：通过第一过滤器过滤样品流体，从而将微物体累积在第一过滤器上。 还公开了一种用于分析含有机微物体的样品流体的装置或系统。

公开(公告)号：US09128016B2

公开(公告)日：2015-09-08

申请号：US13263997

申请日：2010-04-14

申请人： Bas Hulsken ,  Stein Kuiper ,  Sjoerd Stallinga ,  Bo Joakim Isaksson ,  Mark W. G. Ponjee ,  Bart E. G. J. Van Meerbergen ,  Zeynep S. Unay

发明人： Bas Hulsken ,  Stein Kuiper ,  Sjoerd Stallinga ,  Bo Joakim Isaksson ,  Mark W. G. Ponjee ,  Bart E. G. J. Van Meerbergen ,  Zeynep S. Unay

IPC分类号： G01N15/06 ,  G01N1/40

CPC分类号： G01N1/4077 ,  G01N1/4005 ,  G01N15/0625 ,  G01N15/0631

摘要： A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S1) the microobjects by removing, in a total time T1, a volume V1 of the sample fluid from the upconcentrate sample microobjects; immersing (S2) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S3), in a total time T3, a volume V3 of the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S4) an image of the microobjects accumulated on the filter; wherein the throughput V1/T1 of the step of up-concentrating (S1) is greater than the throughput V1/T1, of the step of filtering (S3). The filter may be a second filter, and the step of up-concentrating (S1) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.

摘要翻译： 提出了一种分析含有机微物体样品液的方法。 该方法包括以下步骤：通过在总时间T1中去除来自上升浓度样品微物体的样品流体的体积V1来上升（S1）微观对象; 将微量物体浸入（S2）到转移流体中，或将微量物体留在样品流体的剩余部分中，然后将样品流体的剩余部分提供转移流体; 在总时间T3中过滤（S3）通过过滤器传送流体的体积V3，从而将微物体累积在过滤器上; 以及生成（S4）累积在所述过滤器上的所述微物体的图像; 其特征在于，上述浓缩步骤（S1）的吞吐量V1 / T1大于滤波步骤的吞吐量V1 / T1（S3）。 过滤器可以是第二过滤器，并且上集中（S1）的步骤可以包括：通过第一过滤器过滤样品流体，从而将微物体累积在第一过滤器上。 还公开了一种用于分析含有机微物体的样品流体的装置或系统。