Up-concentration of organic microobjects for microscopic imaging
    1.
    发明授权
    Up-concentration of organic microobjects for microscopic imaging 有权
    用于显微镜成像的有机微量物质的上调

    公开(公告)号:US09128016B2

    公开(公告)日:2015-09-08

    申请号:US13263997

    申请日:2010-04-14

    IPC分类号: G01N15/06 G01N1/40

    摘要: A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S1) the microobjects by removing, in a total time T1, a volume V1 of the sample fluid from the upconcentrate sample microobjects; immersing (S2) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S3), in a total time T3, a volume V3 of the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S4) an image of the microobjects accumulated on the filter; wherein the throughput V1/T1 of the step of up-concentrating (S1) is greater than the throughput V1/T1, of the step of filtering (S3). The filter may be a second filter, and the step of up-concentrating (S1) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.

    摘要翻译: 提出了一种分析含有机微物体样品液的方法。 该方法包括以下步骤:通过在总时间T1中去除来自上升浓度样品微物体的样品流体的体积V1来上升(S1)微观对象; 将微量物体浸入(S2)到转移流体中,或将微量物体留在样品流体的剩余部分中,然后将样品流体的剩余部分提供转移流体; 在总时间T3中过滤(S3)通过过滤器传送流体的体积V3,从而将微物体累积在过滤器上; 以及生成(S4)累积在所述过滤器上的所述微物体的图像; 其特征在于,上述浓缩步骤(S1)的吞吐量V1 / T1大于滤波步骤的吞吐量V1 / T1(S3)。 过滤器可以是第二过滤器,并且上集中(S1)的步骤可以包括:通过第一过滤器过滤样品流体,从而将微物体累积在第一过滤器上。 还公开了一种用于分析含有机微物体的样品流体的装置或系统。

    UP-CONCENTRATION OF ORGANIZ MICROOBJECTS FOR MICROSCOPIC IMAGING
    2.
    发明申请
    UP-CONCENTRATION OF ORGANIZ MICROOBJECTS FOR MICROSCOPIC IMAGING 有权
    用于微观成像的组织微结构的上调

    公开(公告)号:US20120034623A1

    公开(公告)日:2012-02-09

    申请号:US13263997

    申请日:2010-04-14

    IPC分类号: G01N33/566 C12M1/34 C12Q1/02

    摘要: A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S1) the microobjects by removing, in a total time T1, a volume V1 of the sample fluid from the upconcentrate sample microobjects; immersing (S2) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S3), in a total time T3, a volume V3 of the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S4) an image of the microobjects accumulated on the filter; wherein the throughput V1/T1 of the step of up-concentrating (S1) is greater than the throughput V1/T1, of the step of filtering (S3). The filter may be a second filter, and the step of up-concentrating (S1) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.

    摘要翻译: 提出了一种分析含有机微物体样品液的方法。 该方法包括以下步骤:通过在总时间T1中去除来自上升浓度样品微物体的样品流体的体积V1来上升(S1)微观对象; 将微量物体浸入(S2)到转移流体中,或将微量物体留在样品流体的剩余部分中,然后将样品流体的剩余部分提供转移流体; 在总时间T3中过滤(S3)通过过滤器传送流体的体积V3,从而将微物体累积在过滤器上; 以及生成(S4)累积在所述过滤器上的所述微物体的图像; 其特征在于,上述浓缩步骤(S1)的吞吐量V1 / T1大于滤波步骤的吞吐量V1 / T1(S3)。 过滤器可以是第二过滤器,并且上集中(S1)的步骤可以包括:通过第一过滤器过滤样品流体,从而将微物体累积在第一过滤器上。 还公开了一种用于分析含有机微物体的样品流体的装置或系统。

    Birefringent optical system
    6.
    发明授权
    Birefringent optical system 失效
    双折射光学系统

    公开(公告)号:US07440075B2

    公开(公告)日:2008-10-21

    申请号:US10599068

    申请日:2005-03-14

    IPC分类号: G02B27/12

    摘要: The invention relates to an optical system comprising a fluid chamber 1 and a birefringent part. The fluid chamber comprises first and second fluids 10, 12 having different indices of refraction, the interface between the fluids forming a meniscus 14. The birefringent part is capable of varying characteristics of a first radiation beam 3b and a second radiation beam 3c, the first and second radiation beams having different polarisations. Variation in the configuration of the meniscus causes variation in the characteristics of the first radiation beam and the second radiation beam. Variation in the configuration of the meniscus may be controlled by electrowetting.

    摘要翻译: 本发明涉及一种包括流体室1和双折射部分的光学系统。 流体室包括具有不同折射率的第一和第二流体10,12,形成弯液面14的流体之间的界面。 双折射部分能够改变第一辐射束3b和第二辐射束3c的特性,第一和第二辐射束具有不同的极化。 弯液面的构造的变化导致第一辐射束和第二辐射束的特性的变化。 弯液面的构型的变化可以通过电润湿来控制。

    Birefringent Optical System
    7.
    发明申请
    Birefringent Optical System 失效
    双折射光学系统

    公开(公告)号:US20070273943A1

    公开(公告)日:2007-11-29

    申请号:US10599068

    申请日:2005-03-14

    IPC分类号: G02B27/12 G02F1/13 G03H1/22

    摘要: The invention relates to an optical system comprising a fluid chamber 1 and a birefringent part. The fluid chamber comprises first and second fluids 10, 12 having different indices of refraction, the interface between the fluids forming a meniscus 14. The birefringent part is capable of varying characteristics of a first radiation beam 3b and a second radiation beam 3c, the first and second radiation beams having different polarisations. Variation in the configuration of the meniscus causes variation in the characteristics of the first radiation beam and the second radiation beam. Variation in the configuration of the meniscus may be controlled by electrowetting.

    摘要翻译: 本发明涉及一种包括流体室1和双折射部分的光学系统。 流体室包括具有不同折射率的第一和第二流体10,12,形成弯液面14的流体之间的界面。 双折射部分能够改变第一辐射束3b和第二辐射束3c的特性,第一和第二辐射束具有不同的极化。 弯液面的构造的变化导致第一辐射束和第二辐射束的特性的变化。 弯液面的构型的变化可以通过电润湿来控制。

    Spherical Aberration Detector
    10.
    发明申请
    Spherical Aberration Detector 审中-公开
    球面异常检测器

    公开(公告)号:US20080192600A1

    公开(公告)日:2008-08-14

    申请号:US11915633

    申请日:2006-05-22

    IPC分类号: G11B5/58

    摘要: An optical scanning device (1) for scanning at least one information layer (2) of at least one optical record carrier (3). The device includes a radiation source (7) for providing at least a first radiation beam (4) comprising a first wavelength, an objective lens system (8) for converging the first radiation beam on a respective information layer (2), an information detector (23) for detecting at least a portion of the first radiation beam (22) reflected from the respective information layer, for determining information on said layer, and a spherical aberration detection system. The spherical aberration detection system includes an aberration detector (24) for detecting at least a portion of the reflected first radiation beam for determining spherical aberration of the first radiation beam, and a diffractive element (26) for diffracting at least a portion of the reflected first radiation beam towards the aberration detector (24), and for transmitting at least a portion of the reflected first radiation beam towards the information detector (23). In a first mode of operation the grating is arranged to introduce a phase change to an incident portion of a radiation beam for transmitting that portion towards the information detector (23). In a second mode of operation the grating is arranged to introduce a phase change to an incident portion of the reflected first radiation beam for diffracting that portion towards the aberration detector (24).

    摘要翻译: 一种用于扫描至少一个光学记录载体(3)的至少一个信息层(2)的光学扫描装置(1)。 该装置包括用于提供至少包括第一波长的第一辐射束(4)的辐射源(7),用于将第一辐射束会聚在相应的信息层(2)上的物镜系统(8),信息检测器 (23),用于检测从各个信息层反射的第一辐射束(22)的至少一部分,用于确定关于所述层的信息,以及球面像差检测系统。 球面像差检测系统包括:像差检测器(24),用于检测用于确定第一辐射束的球面像差的反射的第一辐射束的至少一部分;以及衍射元件(26),用于衍射至少部分反射 朝向像差检测器(24)的第一辐射束,并且用于将反射的第一辐射束的至少一部分传送到信息检测器(23)。 在第一操作模式中,光栅布置成将相位变化引入辐射束的入射部分,以将该部分传送到信息检测器(23)。 在第二操作模式中,光栅布置成将相变引入到反射的第一辐射束的入射部分,以将该部分衍射到像差检测器(24)。