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公开(公告)号：US10793872B2
公开(公告)日：2020-10-06
申请号：US14441638
申请日：2013-09-13
Applicant: Bayer CropScience AG , Bayer Cropscience LP
Inventor： Fabien Poree , Volker Heinrichs , Gudrun Lange , Bernd Laber , Cheryl Peters , Laura Schouten
Abstract: Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
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公开(公告)号：US20150267180A1
公开(公告)日：2015-09-24
申请号：US14441638
申请日：2013-09-13
Applicant: BAYER CROPSCIENCE LP , BAYER CROPSCIENCE AG
Inventor： Fabien Poree , Volker Heinrichs , Gudrun Lange , Bernd Laber , Cheryl Peters , Laura Schouten
IPC: C12N9/02 , A01N43/647 , A01N43/82 , C12N15/82 , A01N43/80 , A01N43/40 , A01N43/56 , A01N43/713 , A01N35/06
Abstract: Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
Abstract translation: 提供了赋予对细菌，植物，植物细胞，组织和种子的除草剂耐受性的组合物和方法。 组合物包括编码除草剂耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码HPPD抑制剂耐受多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。
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公开(公告)号：US10400249B2
公开(公告)日：2019-09-03
申请号：US15124861
申请日：2015-03-09
Applicant: BAYER CROPSCIENCE AKTIENGESELLSCHAFT , BAYER CROPSCIENCE LP
Inventor： Fabien Poree , Bernd Laber , Gudrun Lange , Manuel Dubald , Roxanne Armstrong
IPC: C12N15/82 , A01N43/713 , C12N9/02
Abstract: In the present invention, HPPD enzymes and plants containing them showing a full tolerance against several classes of HPPD-inhibitors are described.A set of HPPD enzymes have been designed which have either no or only a significantly reduced affinity to HPPD inhibitors and, at the same time, the rate of dissociation of the HPPD inhibitors of the enzyme is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow, tight-binding inhibitors but, instead of this, have become fully reversible inhibitors.In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
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公开(公告)号：US20150159167A1
公开(公告)日：2015-06-11
申请号：US14500716
申请日：2014-09-29
Applicant: Bayer CropScience AG
Inventor： Fabien POREE , Bernd Laber , Nathalie Knittel-Ottleben , Gudrun Lange , Arno Schulz , Ruediger Hain
CPC classification number: C12N15/8274 , C12N9/001 , C12N9/0069 , C12Y103/01012 , C12Y113/11027
Abstract: The present invention relates to nucleic acid sequences encoding a hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, abbreviated herein as HPPD) obtained from bacteria belonging to the subfamily Synechococcoideae, as well as the proteins encoded thereby, and to a chimeric gene which comprises such nucleic acid sequence, and to the use of such nucleic acid sequences, proteins or chimeric genes for obtaining plants which are tolerant to HPPD inhibitor herbicides.
Abstract translation: 本发明涉及从属于亚科孢子虫科的细菌获得的编码羟基苯基丙酮酸双加氧酶（EC 1.13.11.27，本文缩写为HPPD）的核酸序列以及由此编码的蛋白质以及包含该核酸的嵌合基因 序列，以及使用这种核酸序列，蛋白质或嵌合基因来获得对HPPD抑制剂除草剂具有耐受性的植物。
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5.发明申请Mutated Hydroxyphenylpyruvate Dioxygenase, DNA Sequence and Isolation of Plants Which Are Tolerant To HPPD Inhibitor Herbicides 审中-公开突变的羟基苯丙酮酸双加氧酶，DNA序列和耐HPPD抑制剂除草剂植物的分离公开(公告)号：US20140223597A1
公开(公告)日：2014-08-07
申请号：US14256798
申请日：2014-04-18
Applicant: Bayer CropScience AG , Bayer BioScience N.V.
Inventor： Marco Busch , Kerstin Fischer , Bernd Laber , Alain Sailland
CPC classification number: C12N15/8274 , C12N9/0069 , C12Y113/11027
Abstract: The present invention relates to a nucleic acid sequence encoding a mutated hydroxyphenylpyruvate dioxygenase (HPPD), to a chimeric gene which comprises this sequence as the coding sequence, and to its use for obtaining plants which are resistant to HPPD inhibitor herbicides.
Abstract translation: 本发明涉及编码突变型羟基苯基丙酮酸双加氧酶（HPPD）的核酸序列，其涉及包含该序列作为编码序列的嵌合基因，以及其用于获得对HPPD抑制剂除草剂具有抗性的植物的用途。
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Patent Agency Ranking
公开(公告)号：US20150167016A1
公开(公告)日：2015-06-18
申请号：US14507257
申请日：2014-10-06
Applicant: Bayer CropScience AG
Inventor： Fabien POREE , Bernd Laber , Nathalie Knittel-Ottleben , Gudrun Lange , Arno Schulz , Ruediger Hain
CPC classification number: C12N15/8274 , C12N9/0069 , C12Y113/11027
Abstract: The present invention relates to nucleic acid sequences encoding a hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, abbreviated herein as HPPD) obtained from Euryarchaeota belonging to the family Picrophilaceae, as well as the proteins encoded thereby, and to a chimeric gene which comprises such nucleic acid sequence, and to the use of such nucleic acid sequences, proteins or chimeric genes for obtaining plants which are tolerant to HPPD inhibitor herbicides.
Abstract translation: 本发明涉及从属于皮肤科科属的Euryarchaeota获得的编码羟基苯基丙酮酸双加氧酶（EC 1.13.11.27，本文缩写为HPPD）的核酸序列，以及由此编码的蛋白质以及包含该核酸的嵌合基因 序列，以及使用这种核酸序列，蛋白质或嵌合基因来获得对HPPD抑制剂除草剂具有耐受性的植物。
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公开(公告)号：US20150159169A1
公开(公告)日：2015-06-11
申请号：US14512022
申请日：2014-10-10
Applicant: Bayer CropScience AG
Inventor： Fabien POREE , Bernd Laber , Nathalie Knittel-Ottleben , Gudrun Lange , Arno Schulz , Ruediger Hain
CPC classification number: C12N15/8274 , C12N9/001 , C12N9/0069 , C12Y103/01012 , C12Y113/11027
Abstract: present invention relates to nucleic acid sequences encoding a hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, abbreviated herein as HPPD) obtained from bacteria belonging to the genus Kordia, as well as the proteins encoded thereby, and to a chimeric gene which comprises such nucleic acid sequence, and to the use of such nucleic acid sequences, proteins or chimeric genes for obtaining plants which are tolerant to HPPD inhibitor herbicides.
Abstract translation: 本发明涉及从属于科迪亚属的细菌获得的编码羟基苯基丙酮酸双加氧酶（EC 1.13.11.27，本文缩写为HPPD）的核酸序列以及由此编码的蛋白质以及包含该核酸序列的嵌合基因 以及使用这种核酸序列，蛋白质或嵌合基因来获得耐HPPD抑制剂除草剂的植物。
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公开(公告)号：US20150159168A1
公开(公告)日：2015-06-11
申请号：US14507176
申请日：2014-10-06
Applicant: Bayer CropScience AG
Inventor： Fabien POREE , Bernd Laber , Nathalie Knittel-Ottleben , Gudrun Lange , Arno Schulz , Ruediger Hain
IPC: C12N15/82
CPC classification number: C12N15/8274 , C12N9/0069
Abstract: The present invention relates to nucleic acid sequences encoding a hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, abbreviated herein as HPPD) obtained from protists belonging to the family Blepharismidae, as well as the proteins encoded thereby, and to a chimeric gene which comprises such nucleic acid sequence, and to the use of such nucleic acid sequences, proteins or chimeric genes for obtaining plants which are tolerant to HPPD inhibitor herbicides.
Abstract translation: 本发明涉及编码由属于Blepharismidae家族的原生生物获得的羟基苯基丙酮酸双加氧酶（EC 1.13.11.27，本文缩写为HPPD）以及由此编码的蛋白质的核酸序列，以及包含该核酸的嵌合基因 序列，以及使用这种核酸序列，蛋白质或嵌合基因来获得对HPPD抑制剂除草剂具有耐受性的植物。
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公开(公告)号：US20140259213A1
公开(公告)日：2014-09-11
申请号：US13789533
申请日：2013-03-07
Applicant: BAYER CROPSCIENCE AG
Inventor： Gudrun Lange , Fabien Poree , Bernd Laber , Joerg Freigang , Arno Schultz
CPC classification number: C12N15/8274 , C12N9/0008 , C12Y102/03013
Abstract: The present invention relates to an isolated nucleic acid comprising a nucleotide sequence encoding a mutated HPPD protein, wherein said mutated HPPD protein has HPPD activity, wherein in said mutated HPPD protein at least one amino acid has been replaced so that the resulting amino acid sequence comprises at least one amino acid selected from certain amino acids at specific positions important for conferring an increased HPPD inhibitor tolerance. The present invention also relates to proteins encoded by the nucleic acid of the invention, to chimeric genes, plant cells comprising the nucleic acid of the invention operably linked to a plant-expressible promoter and optionally a transcription termination and polyadenylation region, plants essentially consisting of the plant cells of the invention and methods of obtaining transgenic plants.
Abstract translation: 本发明涉及分离的核酸，其包含编码突变的HPPD蛋白的核苷酸序列，其中所述突变的HPPD蛋白具有HPPD活性，其中在所述突变的HPPD蛋白中至少一个氨基酸被替换，使得得到的氨基酸序列包含 至少一种选自特定位置的某些氨基酸的氨基酸，其对于赋予增加的HPPD抑制剂耐受性是重要的。 本发明还涉及由本发明的核酸编码的蛋白质，嵌合基因，包含与植物可表达的启动子和任选的转录终止和多聚腺苷酸化区域可操作地连接的本发明核酸的植物细胞，基本上由 本发明的植物细胞和获得转基因植物的方法。
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公开(公告)号：US20150159145A1
公开(公告)日：2015-06-11
申请号：US14500707
申请日：2014-09-29
Applicant: Bayer CropScience AG
Inventor： Fabien POREE , Bernd Laber , Nathalie Knittel-Ottleben , Gudrun Lange , Arno Schulz , Ruediger Hain
CPC classification number: C12N9/0069 , C12N15/8274 , C12Y113/11027
Abstract: The present invention relates to nucleic acid sequences encoding a hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, abbreviated herein as HPPD) obtained from bacteria belonging to the genus Rhodococcus as well as the proteins encoded thereby, and to a chimeric gene which comprises such nucleic acid sequence, and to the use of such nucleic acid sequences, proteins or chimeric genes for obtaining plants which are tolerant to HPPD inhibitor herbicides.
Abstract translation: 本发明涉及从属于红球菌属的细菌获得的编码羟基苯基丙酮酸双加氧酶（EC 1.13.11.27，本文缩写为HPPD）的核酸序列以及由此编码的蛋白质以及包含这种核酸序列的嵌合基因 以及使用这种核酸序列，蛋白质或嵌合基因来获得耐HPPD抑制剂除草剂的植物。
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