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    HPPD variants and methods of use
    1.
    发明授权
    HPPD variants and methods of use 有权

    公开(公告)号：US10793872B2

    公开(公告)日：2020-10-06

    申请号：US14441638

    申请日：2013-09-13

    Applicant: Bayer CropScience AG ,  Bayer Cropscience LP

    Inventor： Fabien Poree ,  Volker Heinrichs ,  Gudrun Lange ,  Bernd Laber ,  Cheryl Peters ,  Laura Schouten

    IPC: C12N15/82 ,  C12N9/02

    Abstract: Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

    HPPD VARIANTS AND METHODS OF USE
    2.
    发明申请
    HPPD VARIANTS AND METHODS OF USE 审中-公开
    HPPD变量和使用方法

    公开(公告)号：US20150267180A1

    公开(公告)日：2015-09-24

    申请号：US14441638

    申请日：2013-09-13

    Applicant: BAYER CROPSCIENCE LP ,  BAYER CROPSCIENCE AG

    Inventor： Fabien Poree ,  Volker Heinrichs ,  Gudrun Lange ,  Bernd Laber ,  Cheryl Peters ,  Laura Schouten

    IPC: C12N9/02 ,  A01N43/647 ,  A01N43/82 ,  C12N15/82 ,  A01N43/80 ,  A01N43/40 ,  A01N43/56 ,  A01N43/713 ,  A01N35/06

    Abstract: Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

    Abstract translation: 提供了赋予对细菌，植物，植物细胞，组织和种子的除草剂耐受性的组合物和方法。 组合物包括编码除草剂耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码HPPD抑制剂耐受多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。

    HPPD variants and methods of use
    3.
    发明授权
    HPPD variants and methods of use 有权

    公开(公告)号：US10400249B2

    公开(公告)日：2019-09-03

    申请号：US15124861

    申请日：2015-03-09

    Applicant: BAYER CROPSCIENCE AKTIENGESELLSCHAFT ,  BAYER CROPSCIENCE LP

    Inventor： Fabien Poree ,  Bernd Laber ,  Gudrun Lange ,  Manuel Dubald ,  Roxanne Armstrong

    IPC: C12N15/82 ,  A01N43/713 ,  C12N9/02

    Abstract: In the present invention, HPPD enzymes and plants containing them showing a full tolerance against several classes of HPPD-inhibitors are described.A set of HPPD enzymes have been designed which have either no or only a significantly reduced affinity to HPPD inhibitors and, at the same time, the rate of dissociation of the HPPD inhibitors of the enzyme is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow, tight-binding inhibitors but, instead of this, have become fully reversible inhibitors.In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

    HPPD VARIANTS AND METHODS OF USE
    6.
    发明申请
    HPPD VARIANTS AND METHODS OF USE 审中-公开
    HPPD变量和使用方法

    公开(公告)号：US20140259213A1

    公开(公告)日：2014-09-11

    申请号：US13789533

    申请日：2013-03-07

    Applicant: BAYER CROPSCIENCE AG

    Inventor： Gudrun Lange ,  Fabien Poree ,  Bernd Laber ,  Joerg Freigang ,  Arno Schultz

    IPC: C12N15/82 ,  C12N9/02

    CPC classification number: C12N15/8274 ,  C12N9/0008 ,  C12Y102/03013

    Abstract: The present invention relates to an isolated nucleic acid comprising a nucleotide sequence encoding a mutated HPPD protein, wherein said mutated HPPD protein has HPPD activity, wherein in said mutated HPPD protein at least one amino acid has been replaced so that the resulting amino acid sequence comprises at least one amino acid selected from certain amino acids at specific positions important for conferring an increased HPPD inhibitor tolerance. The present invention also relates to proteins encoded by the nucleic acid of the invention, to chimeric genes, plant cells comprising the nucleic acid of the invention operably linked to a plant-expressible promoter and optionally a transcription termination and polyadenylation region, plants essentially consisting of the plant cells of the invention and methods of obtaining transgenic plants.

    Abstract translation: 本发明涉及分离的核酸，其包含编码突变的HPPD蛋白的核苷酸序列，其中所述突变的HPPD蛋白具有HPPD活性，其中在所述突变的HPPD蛋白中至少一个氨基酸被替换，使得得到的氨基酸序列包含 至少一种选自特定位置的某些氨基酸的氨基酸，其对于赋予增加的HPPD抑制剂耐受性是重要的。 本发明还涉及由本发明的核酸编码的蛋白质，嵌合基因，包含与植物可表达的启动子和任选的转录终止和多聚腺苷酸化区域可操作地连接的本发明核酸的植物细胞，基本上由 本发明的植物细胞和获得转基因植物的方法。

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